The Cascada BIO- and LS-water Systems incorporate an ultrafiltration device with a low molecular weight cut off (MWCO). Its role is to reduce bacteria and endotoxins.

When challenged with 2 x 10⁶ CFU/mL of typical waterborne bacteria under standard flow conditions, the ultrafilter in the Cascada BIO- and LS-water Systems produced water with a bacterial content below the detection limit of 0.01 CFU/mL - a log₁₀ reduction of > 8 CFU/mL.

These results demonstrate that even in the extremely unlikely event of a very high level of bacteria reaching the ultrafilter, there would be no breakthrough into the product water.

Sample	Bacteria Concentration
Pre UF	2 x 107 CFU/mL
Post UF 1 minute	< 0.01 CFU/mL
Post UF 2 minutes	< 0.01 CFU/mL
Post UF 3 minutes	< 0.01 CFU/mL
Post UF 4 minutes	< 0.01 CFU/mL
Post UF 5 minutes	< 0.01 CFU/mL
Log10 Reduction	> 8 CFU/mL

A challenge was prepared by inoculating 200 mL of sterile peptone water with 10 mL of purified water and agitating and incubating for 2 days at 27 °C to produce a culture of bacteria commonly found in purified water. After dilution to 25 liters, the water contained 2 x 10⁶ CFU/mL.

This challenge was pumped through the ultrafiltration device under normal operating conditions. Samples were taken before and after the ultrafiltration device. The bacteria present were measured by filtration and incubation on R2A agar at 27 °C for 5 days.