 |
|
||||
 |
Cascada™ BIO-, AN-, LS-, and IX-Water Systems: Point-of-Use - Bacteria and Endotoxin Release |
Introduction
The Cascada Lab Water Systems can incorporate a 0.2 µm point-of-use (POU) filter to remove bacteria from the product water. It is important that the POU filter does not add significant levels of impurities to the water. A new filter was tested for bacteria and endotoxin release.
No bacteria could be detected and only an initial trace of endotoxin was found, which rinsed away within 1 minute.
Top
Results
Sample Time Bacteria TVC Before POU filter < 0.01 CFU/mL After POU filter 0 sec < 0.01 CFU/mL After POU filter 20 sec < 0.01 CFU/mL After POU filter 1 min < 0.01 CFU/mL After POU filter 2 min < 0.01 CFU/mL After POU filter 4 min < 0.01 CFU/mL
Sample Time Endotoxin Before POU filter < 0.001 (EU/mL) After POU filter 0 sec 0.007 (EU/mL) After POU filter 20 sec 0.07 (EU/mL) After POU filter 1 min < 0.001 (EU/mL) After POU filter 2 min < 0.001 (EU/mL) After POU filter 4 min < 0.001 (EU/mL)
Top
Method
A POU filter was fed with ultrapure water at 1 L/min and samples taken under sterile conditions before and after the filter. Samples were analyzed for bacteria by membrane filtration technique and incubation on R2A agar at 27 °C for 5 days. Endotoxin assay used an Ati 320 kinetic LAL analyzer.
Top