Resolute® Chromatography Columns

Resolute columns are ideally suited for process purification in the biopharmaceutical industry. A patented nozzle valve provides all column functions required for packing, unpacking and running of the column in a closed system. Based on established and well proven designs, the Resolute column provides improved column performance and true linear scalability combined with reproducible column packing methods for today’s high performance media. Pall continues to work closely with the biopharmaceutical manufacturing industry to advance the state of the art for both chromatographic performance and productivity. The packing process is controlled by a slurry packing system which,

combined with a Resolute column, offers a complete solution for process chromatography from development to manufacturing scale.  Features and Benefits Standard column diameters from 280 mm to 1200 mm – all with selectable bed heights from  100 mm up to 600 mm   Bed Height: fixed or adjustable (200 mm adjustment) Nozzle Valve: choice of manually operated Resolute DM, or pneumatically actuated Resolute DP columns Choice of bed supports in polyethylene or stainless steel Piston seal and precision bore tube eliminates need for additional mechanical compression or pneumatic activation of adjuster seals Compatible with a wide range of Chromatographic Media

Sanitary Design

Fully flushed flow path and adjuster seal for clean-in-place (CIP) Minimum dead space fixed cell seal arrangement Reduced risk of corrosion non metallic nozzle flow path for high  salt and low pH conditions forged stainless steel tube eliminates weld seams on tube wall and flanges Visible valve flow path aids detection of entrapped air Sanitary clamp terminations Leachate free acrylic tube — no phthalates Peroxide cured seals — no sulphur containing leachate Process wetted materials meet regulatory requirements

Column Options

Standard Columns

• Actuated nozzle valve; fits 400 to 1200 mm diameter columns
• Bed supports:
  • Polyethylene 10/20/60 µm
  • Stainless steel 10/20/50 µm
• Lockable castors and adjustable feet supplied as standard*
• Up to 1000 mm diameter only.

Engineered Columns

• Operating pressure up to 10 bar
• Columns from 400 mm to 2000 mm diameter
• Certification to ASME Div VIII sec 1 or PD5500.CE where applicable
• Service frames for piston end cell maintenance
• Alternative materials: Hastalloy C22, Stainless Steel 1.4435, 1.4439
• Hydraulic Actuators — see technical specifications for operating modes

View additional chromatography columns.

Products in this datasheet may be covered by one or more patents including :
EP 1 085 931
US 6,446,679

Material Specifications

Process Wetted Components
Column Tube	Acrylic (cast PMMA) or Stainless Steel 316L (1.4404)
Distribution Cell	Polypropylene
Nozzle Body	PVDF/Acrylic(1)
Process Terminations and Slurry Nozzle Tip	PEEK
Slurry Inlet Port	180 and 280 mm columns: (2) PEEK 400 to 1200 mm columns: (2) Stainless Steel 316L
Bed Support	Polyethylene sinter or Stainless Steel mesh
Seals	EPDM (Peroxide cured) FEP encapsulated silicone
Wiper Blade	PTFE

External Components
Stand, Adjustable Feet	Stainless Steel 316L
Castor	Stainless Steel 304L
Castor Tyre	Polyurethane

⁽¹⁾ PVDF/PVDF version available for increased chemical resistance.
⁽²⁾ Components not in mobile phase flow path.

Column Specifications

Resolute Fixed and Adjustable Columns 180 – 1200 mm (7 – 47 in.) diameter
Operating Pressure	180 – 280 mm (7 – 11 in.):  5 bar (73) psi 400 – 1200 mm (15.7 – 47 in.): 3 bar (44) psi
Operating Temperature	2 – 30 ºC (35 – 86 °F)
Bed Support, Type and Rating	Stainless Steel Mesh: 10, 20, 50 µm Polyethylene Sinter: 10, 20, 60 µm
Product Flow Path	Stainless Steel surface finish < 0.6 µm Ra, Electropolished
Exterior Components	Stainless Steel surface finish < 0.9 µm Ra, Electropolished
Pressure Retaining Plates	Stainless Steel surface finish < 1.5 µm Ra, 240 (UK) Grit Sateen
Column Frame	Stainless Steel surface finish Bright polished
Media Transfer Nozzle	DM: Manual actuation DP: Manual control with pneumatic actuation

Hydraulic Actuators (Optional)

Type	Mode of Operation	Application
HEP Hydraulic Endcell Positioning	Hydraulic linear actuator when column is empty or primed and has an open flow path with < 1 bar column pressure	Set up prior to Pack in Place and Maintenance
DAP Dynamic Axial Packing	Hydraulic linear actuator when column is empty or filled with slurry and has an open flow path with < 3.0 bar column pressure	Flow Packing at optimal linear velocity
/M Option Hoist-free Maintenance	Actuators and integral support and safety devices move and secure Top Adjuster and Column Tube (/M option available for either HEP or DAP model)	Access to top and bottom bed supports and seals for changeout

This is a list of typical part numbers for this product range. For part numbers and configurations that are that are not listed, please contact your Pall representative.

Column Capacity and Ordering Information

Description	Diameter	CSA	Adjustable Height	Adjustable Capacity	Operating Pressure
Resolute 280	280 mm (11.in.)	620 cm2	100 – 300 mm	6.2 – 18.5 L	5 bar (73 psi)
Resolute 400	400 mm (15.7 in.)	1260 cm2	100 – 300 mm	12.6 – 37.7 L	3 bar (44 psi)
Resolute 600	600 mm (23.6 in.)	2830 cm2	100 – 300 mm	28.3 – 84.8 L	3 bar (44 psi)
Resolute 800	800 mm (2.6 ft)	5030 cm2	100 – 300 mm	50.3 – 150 L	3 bar (44 psi)
Resolute 1000	1000 mm (3.2 ft)	7850 cm2	100 – 300 mm	79 – 235 L	3 bar (44 psi)
Resolute 1200	1200 mm (3.9 ft)	11310 cm2	100 – 300 mm	113 – 339 L	3 bar (44 psi)
Resolute 1400	1400 mm (4.6 ft)	15390 cm2	100 – 300 mm	154 – 461 L	3 bar (44 psi)
Resolute 1600	1600 mm (5.2 ft)	20110 cm2	100 – 300 mm	201 – 603 L	3 bar (44 psi)
Resolute 1800	1800 mm (5.9 ft)	25450 cm2	100 – 300 mm	255 – 763 L	3 bar (44 psi)
Resolute 2000	2000 mm (6.6 ft)	31420 cm2	100 – 300 mm	314 – 942 L	3 bar (44 psi)