Prefiltration and Clarification for Proteomics Sample Prep

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Although a basic filtration concept, the clarification and prefiltration of samples remains an important function within proteomics. When filtration is used as a prefilter, matching the proper filter media and device to the application is critical. Here, larger pore size filter materials are used to filter solutions prior to more detailed analysis. When selecting the best product for your application, numerous factors need to be considered. Sample viscosity, sample volume and sample recovery are just some of the aspects that will drive the selection of the optimal device. This chart highlights some of our recommended part numbers for proteomics sample prep.

Sample Volume (dependent on serum concentration)	Device	Sample Viscosity Lower	Sample Viscosity Higher	Sample Viscosity Highest
< 5 L	VacuCap® PF Bottle-top Filter	4628 4638
< 150 mL	Serum Acrodisc® Syringe Filter		4525
< 150 mL	Acrodisc PSF Syringe Filter			AP-4523
< 100 mL	Acrodisc PF Syringe Filter	4658 4187
< 1 mL	AcroPrep™ 96 Filter Plate		5053 5046	5041

Literature and Technical Support

• Technical Report, Lysate Clearance for Prokaryotic DNA Isolation Using the AcroPrep 96 Filter Plate
• Technical Report, Syringe Filter Efficiency and Effect of Filtration on HPLC Column Life
• Brochure, Improve Biopurification Processes

See also, information about products for non-critical, coarse filtration.