| Tangential flow filtration (TFF) is a rapid and efficient method for separation and purification of biomolecules. It can be applied to a wide range of biological fields such as immunology, protein chemistry, molecular biology, biochemistry, and microbiology. TFF can be used to concentrate and desalt sample solutions ranging in volume from 10 milliliters to thousands of liters. It can be used to fractionate large from small biomolecules, harvest cell suspensions, and clarify fermentation broths and cell lysates.
In TFF, liquid is pumped across the membrane surface, minimizing fouling by sweeping retained molecules off the surface. Filtration is achieved by creating pressure against the membrane in the retentate stream, causing solute and small molecules to pass through the membrane. TFF is used in larger-scale laboratory separations such as cell harvesting or protein isolation from culture supernatants. It is also commonly used in pilot-scale industrial operations and scaled up to full production.
Learn how to choose the proper TFF system for your application.
Increase Productivity Using the Minimate Capsule
The Minimate™ TFF system and Minimate capsule work together to process sample volumes up to 1 liter and efficiently concentrate samples to as little as 5 mL. To begin processing, simply plug a Minimate capsule into a Minimate system, add sample and turn on the pump. Subsequent desalting or buffer exchange steps can be run on the same system with minimal user intervention. Read more... Top |
