Vivid™ 170 Nitrocellulose Membrane

For Lateral Flow Diagnostics

    

Nitrocellulose membranes are the heart of lateral flow assays and require highly consistent measurements for both wick rate and thickness. Variations in both thickness and wick rates can have detrimental effects to assay sensitivity, reproducibility, and reagent consumption. We are proud to introduce our newest membrane for lateral flow diagnostics, the Vivid 170 nitrocellulose membrane. At Pall, we understand the key consistency measures effecting lateral flow assay development and test strip manufacture and have developed our membrane with tight Coefficients of Variation (CV’s) for the critical membrane parameters. We have demonstrated CV’s of 5% on thickness and 10% for wick rates and protein binding. All of the consistency performance measures evaluated fall within a +/-3 sigma interval indicating a capable and robust manufacturing process. These tight consistency measurements result in highly sensitive membrane even at dilute concentrations of target analytes.

Vivid nitrocellulose membrane is cast on a clear polyester backing without the use of adhesives which can leach into the membrane and impede the performance of the assay. When cast, nitrocellulose membranes traditionally lack the tensile strength needed for large scale manufacturing. The polyester backing provides rigidity and ease of handling of the membrane. The crisp white nature of the membrane results in clear sharp capture lines with minimal background resulting in improved visualization of assay results.

Typical Membrane Characteristics

Base Material	Backing	Thickness (mils)	Thickness (µm)	Tensile Strength*
Nitrocellulose	Polyester	7.28 +/- 0.79	185 +/- 20.0	> 12 N

*Measured on 15 x 1000 mm strip using DIN 53 112, part 1.

Typical Performance Characteristics

Base Material	Capillary Speed (Water) (sec/4 cm)	Protein Binding Capacity
Nitrocellulose	150-225	45-59 µg/cm2 of BSA

Applications

• Lateral flow diagnostics
• Reaction membrane

Sealing

• Mechanical
• Adhesive
• Insert molding

Vivid 170 Nitrocellulose Membrane Provides Increased Sensitivity

Vivid 170 Nitrocellulose Membrane Exhibits Highly Consistent Assay Performance

Vivid 170 Nitrocellulose Membrane Exhibits More Consistent BSA Binding Than Competitive Membranes

Vivid 170 Nitrocellulose Membrane Thickness CV’s are Tightly Controlled

• Vivid Plasma Separation Membrane provides a onestep separation of plasma from whole blood. Plasma yields are > 80% with low hemolyis in less than 2 minutes.
• White Blood Cell Isolation Medium isolates leukocytes from whole blood samples. The nucleic acid content can be extracted for further analysis in molecular detection applications.
• Conjugate Pads are available in a variety of thicknesses and materials of construction to optimize assay performance.
• Absorbent Pads constructed of pure cellulose fibers are available in a variety of absorbencies depending on sample type and volume.

Custom roll, sheet, and disc sizes available upon request. Please contact your local sales representative for additional information.

Part Number	Description	Pkg
VIV170SAMP	Vivid 170 Nitrocellulose membrane, 25 mm x 300 mm strip	1/pkg
VIV1702503R	Vivid 170 Nitrocellulose membrane, 25 mm x 3 m roll	1/pkg
VIV1702550R	Vivid 170 Nitrocellulose membrane, 25 mm x 50 m roll	1/pkg