Introduction
SDR HyperD® solvent-detergent removal
chromatography sorbent is a unique sorbent designed to eliminate solvent and detergent from biological fluids.
It combines a silica-bead moiety filled with a three-dimensional cross-linked hydrophobic polymer. The particle size distribution (40-100 µm), the small pore size of the silica beads and the nature of the polymer have been optimized for a retention of solvents and detergents typically used in viral inactivation processes (i.e. Tri-n-Butyl Phosphate – TnBP – , Triton X-45 and Triton X-100).
The adsorption mechanism involves both the silica moiety and the hydrophobic polymer. The adsorption of Triton X-100 is proportional to the silica surface area, whereas the adsorption of TnBP is linked to binding to the organic polymer moiety. Large molecules are excluded.
Features and Benefits
- Efficient solvent and detergent removal from plasma fractions
- Easier to handle compared to conventional oil extraction or C18 chromatography methods
- Detergent removal from biological samples
- Rigid and mechanically stable for process scale
Sorbent Specifications
|
| Sorbent Structure |
Spherical silica beads filled with a three-dimensional hydrophobic polymer |
| Average Particle Size |
40 – 100 µm |
| Binding Capacity for Triton X100* |
≥90 mg/mL |
| Typical Sample Load |
2 – 3 times the column volume with residence times of5 min using IgG or ATIII treated solutions |
| Recommended Residence Time |
5 – 15 min |
| Operating pH Range |
2 – 12 |
| Sorbent Pressure Resistance |
70 bar (1000 psi) |
|
* Determined using 5 mg/mL Triton X-100 in PBS, pH 7.4, 10% breakthrough, 300 cm/h
Sorbent Ordering Information
|
| 25 mL |
20033-031 |
| 100 mL |
20033-023 |
| 1 L |
20033-015 |
| 5 L |
20033-056 |
| 10 L |
20033-049 |
|
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