Mustang® Chromatography Capsules and Cartridges
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Combining high-capacity, ion-exchange Mustang membranes with a unique 16-layer open-pleat design results in a high-flow rate, high-capacity, single-use, versatile range of pleated products that can be used for a wide range of applications.
This 16-layer construction is held constant from the laboratory-scale Mustang Coin Unit to the largest industrial process-scale capsule, ensuring constant chromatography bed depth in all formats. Mustang E capsules use three layers of a standard pleat format membrane to remove endotoxin from process feedstreams. The three-layer construction is held constant from the Mustang E Acrodisc® format to the largest industrial process scale capsule.
The ease of linear scale-up ensures a shortened process development time by significantly reducing the re-optimization required between scale-up steps. These units are specifically designed for single-use. This eliminates cleaning and the costs associated with cleaning validation. Mustang capsules are fully self-contained units that connect directly into a system using 18 mm (1.5 in.) sanitary flange connectors. Mustang cartridges require the use of a Code 7 housing which requires cleaning and reassembly after each use.
This 16-layer construction is held constant from the laboratory-scale Mustang Coin Unit to the largest industrial process-scale capsule, ensuring constant chromatography bed depth in all formats. Mustang E capsules use three layers of a standard pleat format membrane to remove endotoxin from process feedstreams. The three-layer construction is held constant from the Mustang E Acrodisc® format to the largest industrial process scale capsule.
The ease of linear scale-up ensures a shortened process development time by significantly reducing the re-optimization required between scale-up steps. These units are specifically designed for single-use. This eliminates cleaning and the costs associated with cleaning validation. Mustang capsules are fully self-contained units that connect directly into a system using 18 mm (1.5 in.) sanitary flange connectors. Mustang cartridges require the use of a Code 7 housing which requires cleaning and reassembly after each use.
Mustang Q Membrane
Mustang Q membrane is an anion-exchange support with pendant quaternary amine functional groups in a cross-linked polymeric coating on a 0.8 micron pore-size membrane. This gives high dynamic capacities for high molecular weight products such as DNA, plasmids or even particles as large as viruses.Mustang S Membrane
Mustang S membrane is a cation-exchange support with pendant sulfonic functional groups in a cross-linked polymeric coating on a 0.8 micron pore-size membrane. This gives high dynamic capacities for high molecular weight products such as IgG, Factor VIII and some viruses.Mustang E Membrane
Mustang E membrane is a highly cross-linked quaternized amine charge polymer coating on a 0.2 micron pore size membrane. This gives very high dynamic capacities, under selected conditions for the removal of endotoxin from process feedstreams, buffers and water.
Features and Benefits
- Binding Efficiency: charged biomolecules are readily bound in a single pass
- Speed: high-flow rates enable the processing of large volumes in less than a single working shift. Mustang units typically operate at flow rates between 10 to 40 membrane volumes per minute, making them much faster than conventional columns which typically operate at 0.5 column volumes per minute
- Scalability: full range of sizes accommodates the different volumes and capacities required in biopharmaceutical processing
- Convenience: ready to use, autoclavable and disposable to eliminate potential packing, cleaning, validation and cross-contamination issues
- Flexible: available in capsule or cartridge format
- Cost: lower operating costs and capital investment compared to conventional columns that need validated packing and cleaning
High Quality Standards
- Manufactured to high quality assurance standards in accordance with ISO 9000
- Membrane lots tested for dynamic protein binding capacity, and peak position using standard proteins
- Identified by lot number and unique serial number for complete traceability of manufacturing history, satisfying stringent QC/QA requirements
- Supplied with Certificate of Analysis to confirm quality control
- Meets USP Biological reactivity tests in vivo in accordance with USP Class VI 50 ºC and all materials listed in Drug Master File submitted to the FDA
Comprehensive Validation
- Extensive validation to ensure consistent and reliable performance
- A comprehensive validation guide is available for each of the membrane chemistries on request
Products in this datasheet may be covered by one or more patents including :
EP 1 189 685
US 6,849,185
US 7,189,322
Mustang Capsules
Materials of Construction
| Item | CLxMSTG*P1 | NPxMSTG*P1 |
| Membrane | Modified hydrophilic polyethersulfone | Modified hydrophilic polyethersulfone |
| Membrane Support and Drainage Layer Assembly | Polypropylene | Polypropylene |
| Core and Cage | Polypropylene | Polypropylene |
| Endcaps and Adapters | Polyester | Polyester |
| Housing Bowl | Polyetherimide | Polypropylene |
| Housing Head | Polyetherimide with TiO2 | Polypropylene with TiO2 |
| O-rings | Silicone elastomer | Silicone elastomer |
For CLxMST*P1, x =
M05 for a Q, S or E unit with a bed volume of 10 mL
3 for a Q or S unit with a bed volume of 60 mL or an E unit with a bed volume of 40 mL
For NPxMSTG*P1, x =
6 for a Q or S unit with a bed volume of 260 mL or an E unit with a bed volume of 160 mL
7 for a Q or S unit with a bed volume of 520 mL or an E unit with a bed volume of 320 mL
8 for a Q or S unit with a bed volume of 780 mL or an E unit with a bed volume of 480 mL
* =
Q for a unit with Q (quaternary amine) chemistry membrane
S for a unit with S (sulfonic acid) chemistry membrane
E for a unit with E (polyethyleneimine) chemistry membrane
Nominal Dimensions
| Capsule Type | CLM05MSTG*P1 | CL3MSTG*P1 | NP6MPSTG*P1 | NP7MSTG*P1 | NP8MSTG*P1 |
| Maximum Diameter (inc. Valves) | 123 mm (4.8 in.) | 123 mm (4.8 in.) | 154 mm (6.1 in.) | 154 mm (6.1 in.) | 154 mm (6.1 in.) |
| Length with Sanitary Flange Fittings | 84 mm (3.3 in.) | 157 mm (6.2 in.) | 335 mm (13.2 in.) | 581 mm (22. 9 in.) | 831 mm (32.7 in.) |
| Bed Volume | 10 mL | Q and S = 60 mL E = 40 mL |
Q and S = 260 mL E = 160 mL |
Q and S = 520 mL E = 320 mL |
Q and S = 780 mL E = 480 mL |
Operating Characteristics¹
| Maximum Operating Pressure; Maximum Differential Pressure | 4.1 bar (59.5 psi) at 38 °C |
| Maximum Sanitization Conditions | 1 N NaOH for 30 minutes for one cycle only |
| Maximum Autoclave Conditions | 121° C for 30 minutes for one cycle only |
Typical Binding Characteristics
| Chemistry | Molecule | MW or Size | CLM05 | CL3 | NP6 | NP7 | NP8 |
| Q | BSA | 65 kD | 0.5 – 0.6 g | 3.0 – 3.6 g | 13 – 16 g | 26 – 31 g | 39 – 47 g |
| Q | Thyroglobulin | 650 kD | 0.3 g | 1.8 g | 7.8 g | 15.6 g | 23.4 g |
| Q | DNA | - | 0.20 – 0.25 g | 1.2 – 1.5 g | 5.2 – 6.5 g | 10.4 – 13.0 g | 15.6 – 19.5 g |
| Q | Plasmid DNA | 4.5 kb plasmid | 0.15 g | 0.9 g | 3.9 g | 7.8 g | 11.7 g |
| Q | Plasmid DNA | 12 kb plasmid | 0.15 g | 0.9 g | 3.9 g | 7.8 g | 11.7 g |
| Q | Adenovirus | 70 – 90 nm | 5 x 1013 viral particles | 3 x 1014 viral particles | 1.3 x 1015 viral particles | 2.6 x 1015 viral particles | 3.9 x 1015 viral particles |
| S | Lysozyme | 14.3 kD | 0.45 – 0.50 g | 2.7 – 3.0 g | 11.7 – 13.0 g | 23.4 – 26.0 g | 35.1 – 39.0 g |
| S | Human IgG | 160 kD | 0.2 g | 1.2 g | 5.2 g | 10.4 g | 15.6 g |
| E | Endotoxin Units | 4 x 107 EU/capsule | 1.6 x 108 EU/capsule | 6.4 x 108 EU/capsule | 1.28 x 109 EU/capsule | 1.92 x 109 EU/capsule | |
Mustang Cartridges
Materials of Construction
| Item | ABxMSTG*7PH4 |
| Membrane | Modified hydrophilic polyethersulfone |
| Membrane Support and Drainage Layer Assembly | Polypropylene |
| Core and Cage | Polypropylene |
| Endcaps, Adapters and Bomb Fins | Polyester |
| O-rings | Silicone elastomer |
2 for a 508 mm (20 in.) Q or S unit with a bed volume of 520 mL
3 for a 762 mm (30 in.) Q or S unit with a bed volume of 780 mL
* = Q for a unit with Q (quaternary amine) chemistry membrane
S for a unit with S (sulfonic acid) chemistry membrane
Operating Characteristics²
| Maximum Operating Pressure | 3 bar (43.5 psi) at 38 °C |
| Maximum Differential Pressure | 3 bar (43.5 psi) at 38 °C |
| Maximum Sanitization Conditions | Conditions 1 N NaOH for 30 minutes for one cycle only |
| Maximum Autoclave Conditions | 121 °C for 30 minutes one cycle only |
Nominal Dimensions
| Cartridge Type | Diameter | Length | Bed Volume |
| AB1MSTG*7PH4 | 70 mm (2.75 in.) | 254 mm (10 in.) | 260 mL |
| AB2MSTG*7PH4 | 70 mm (2.75 in.) | 508 mm (20 in.) | 520 mL |
| AB3MSTG*7PH4 | 70mm (2.75 in.) | 762 mm (30 in.) | 780 mL |
Typical Binding Characteristics
| Chemistry | Molecule | MW or Size | AB1 | AB2 | AB3 |
| Q | BSA | 65 kD | 13 – 16 g | 26 – 31 g | 39 – 47 g |
| Q | Thyroglobulin | 650 kD | 7.8 g | 15.6 g | 23.4 g |
| Q | DNA | - | 5.2 – 6.5 g | 10.4 – 13.0 g | 15.6 – 19.5 g |
| Q | Plasmid DNA | 4.5 kb plasmid | 3.9 g | 7.8 g | 11.7 g |
| Q | Plasmid DNA | 12 kb plasmid | 3.9 g | 7.8 g | 11.7 g |
| Q | Adenovirus | 70 – 90 nm | 1.3 x 1015 viral particles | 2.6 x 1015 viral particles | 3.9 x 1015 viral particles |
| S | Lysozyme | 14.3 kD | 11.7 – 13.0 g | 23.4 – 26.0 g | 35.1 – 39.0 g |
| S | Human IgG | 160 kD | 5.2 g | 10.4 g | 15.6 g |
Mustang Capsules
| Chemistry | Bed Volume | Pack Quantity | Part Number | Description |
| Q | 10 mL | 1 | CLM05MSTGQP1 | 38 mm (1.5 in.) sanitary connectors, in-line capsule |
| Q | 60 mL | 1 | CL3MSTGQP1 | 38 mm (1.5 in.) sanitary connectors, in-line capsule |
| Q | 260 mL | 1 | NP6MSTGQP1 | 38 mm (1.5 in.) sanitary connectors, in-line capsule |
| Q | 260 mL | 1 | NT6MSTGQP1 | 38 mm (1.5 in.) sanitary connectors, T-style capsule |
| Q | 520 mL | 1 | NP7MSTGQP1 | 38 mm (1.5 in.) sanitary connectors, in-line capsule |
| Q | 520 mL | 1 | NT7MSTGQP1 | 38 mm (1.5 in.) sanitary connectors, T-style capsule |
| Q | 780 mL | 1 | NP8MSTGQP1 | 38 mm (1.5 in.) sanitary connectors, in-line capsule |
| S | 10 mL | 1 | CLM05MSTGSP1 | 38 mm (1.5 in.) sanitary connectors, in-line capsule |
|
S |
60 mL | 1 | CL3MSTGSP1 | 38 mm (1.5 in.) sanitary connectors, in-line capsule |
| S | 260 mL | 1 | NP6MSTGSP1 | 38 mm (1.5 in.) sanitary connectors, in-line capsule |
| S | 520 mL | 1 | NP7MSTGSP1 | 38 mm (1.5 in.) sanitary connectors, in-line capsule |
| S | 780 mL | 1 | NP8MSTGSP1 | 38 mm (1.5 in.) sanitary connectors, in-line capsule |
| E | 10 mL | 1 | CLM05MSTGEP1 | 38 mm (1.5 in.) sanitary connectors, in-line capsule |
| E | 40 mL | 1 | CL3MSTGEP1 | 38 mm (1.5 in.) sanitary connectors, in-line capsule |
| E | 160 mL | 1 | NP6MSTGEP1 | 38 mm (1.5 in.) sanitary connectors, in-line capsule |
| E | 320 mL | 1 | NP7MSTGEP1 | 38 mm (1.5 in.) sanitary connectors, in-line capsule |
| E | 480 mL | 1 | NP8MSTGEP1 | 38 mm (1.5 in.) sanitary connectors, in-line capsule |
Mustang Cartridges
| Chemistry | Bed Volume | Pack Quantity | Part Number | Description |
| Q | 260 mL | 1 | AB1MSTGQ7PH4 | 254 mm (10 in.) cartridge element with Code 7 adapter |
| Q | 520 mL | 1 | AB2MSTGQ7PH4 | 508 mm (20 in.) cartridge element with Code 7 adapter |
| Q | 780 mL | 1 | AB3MSTGQ7PH4 | 762 mm (30 in.) cartridge element with Code 7 adapter |
| S | 260 mL | 1 | AB1MSTGS7PH4 | 254 mm (10 in.) cartridge element with Code 7 adapter |
| S | 520 mL | 1 | AB2MSTGS7PH4 | 508 mm (20 in.) cartridge element with Code 7 adapter |
| S | 780 mL | 1 | AB3MSTGS7PH4 | 762 mm (30 in.) cartridge element with Code 7 adapter |