PRC Prepacked Chromatography Columns with MEP, HEA, and PPA HyperCel™ Mixed-mode Sorbents

Rapid selectivity screening of MEP, HEA, and PPA HyperCel mixed-mode sorbents

• Convenient – Ready-to-use 1 mL and 5 mL prepacked columns.
• Easy to use – Direct connection to commonly-used laboratory chromatography systems such as ÄKTA* systems.
• Efficient – High packing efficiency (> 2,500 plates/meter).
• Consistent – Screen Pall mixed-mode chromatography sorbents under reliable and reproducible conditions, and guarantee performance run after run.

Mixed-mode Chromatography Sorbents for Antibody Capture and “No Salt” Hydrophobic Interaction

MEP, HEA and PPA HyperCel sorbents exploit unique selectivities of robust synthetic ligands to capture proteins or separate them from contaminants. They provide a unique separation mechanism different from conventional methods. All ligands operate predominantly by hydrophobic interaction. This “HIC-like” interaction typically takes place without the addition of lyotropic salt.

*ÄKTA is a trademark of GE Healthcare companies.

Rapid screening and condition optimization in the 1 mL PRC column enables rapid selection of the appropriate chemistry. Once the chemistry is selected on a 1 mL PRC column, the conditions of use can be optimized in a 5 mL PRC column in doubling the height.

Two or more columns can be connected in series to increase the column height and more closely model real conditions in pilot scale or for scale down applications. Further scale up can be achieved with minimal re-optimization by packing Pall LRC empty glass columns.

Mixed-mode Chromatography Sorbents for Antibody Capture and “No Salt” Hydrophobic Interaction

• MEP HyperCel sorbent: Antibody capture; alternative to conventional hydrophobic interaction
• MEP, HEA, PPA HyperCel sorbents: No salt/low salt alternative to hydrophobic interaction

Sorbent Specifications

Volume
1 mL (5 mm ID x 50 mm)
5 mL (8 mm ID x 100 mm)

Functionalities
Mixed-mode: MEP, HEA and PPA HyperCel sorbents

Storage Solution
30% isopropanol/100 mM sodium phosphate, pH 4.3

Working Pressure¹
< 0.5 barg (7 psig)

Column Specifications

Outer Dimensions
10 x 100 mm for 1 mL columns
11.5 x 140 mm for 5 mL columns

Materials of Construction
Body and end caps: Molded polypropylene
17 µm frit: PP/PE (Polypropylene/Polyethylene)

Connections²
Built-in 10-32 fittings

Maximum Operating Pressure
20 barg (290 psig) for 1 mL columns
30 barg (435 psig) for 5 mL columns

¹ Pressure at 600 cm/h equivalent to 2 mL/min in 0.1 M NaCl.

² For HPLC/MPLC/AKTA systems, direct connection with 1/16 in. tubing and 10-32 fittings. For connecting to systems with M6 or 1/4-28 fittings, consult the appropriate system manual for necessary fittings and adapters.

Mixed-mode Sorbent	Ligand	Average Particle Size (µm)	Ligand Density (µmole/mL)	Dynamic Capacity (mg/mL)1
MEP HyperCel	4-mercapto-ethyl-pyridine (pKa = 4.8)	90	80 – 125	> 202
HEA HyperCel	Hexylamine (aliphatic) (pKa = 8.0)	90	58 – 84	> 403
PPA HyperCel	Phenylpropylamine (aromatic) (pKa = 8.0)	90	58 – 80	> 403

¹ Determined at 10% breakthrough using:
² 5 mg/mL human IgG in PBS, flow rate 60 cm/h
³ 5 mg/mL BSA in PBS, flow rate 100 cm/h

Separation of Lysozyme, α-Chymotrypsinogen, Ovalbumin and BSA on a Pall PRC PPA HyperCel Prepacked Column

Column: Pall PRC prepacked column PPA HyperCel, 5 mm I.D. x 50 mm, volume: 1 mL, linear flow rate: 75 cm/h. Equilibration and wash: PBS, pH 7.4. Load: 500 µL (lysozyme, α-chymotrypsinogen, ovalbumin and BSA, all at 2 mg/mL in equilibration buffer). Elution: by pH step gradient in phosphate/citrate buffer, pH 7.0 - 5.4 and 2.6.

Pall offers a comprehensive line of chromatography columns from lab to process scale.

	PRC Columns 1 - 5 mL (prepacked) Fast selectivity screening with Q and S HyperCel Ion Exchange Sorbents with Q and CM Ceramic HyperD Ion Exchange Sorbents with MEP, HEA, and PPA HyperCel Mixed-mode Sorbents
	LRC Columns 5 - 900 mL Preparative and process optimization
	Resolute® Columns 6 - 300 L Pilot and small-scale production
	Resolute Columns 50 - 1500 L Large-scale production

PRC Prepacked Sorbent Ordering Information

Part Number	Description	Packaging
PRC05X050MEPHCEL01	PRC Column 5x50 MEP HyperCel	1 mL
PRC05X050HEAHCEL01	PRC Column 5x50 HEA HyperCel	1 mL
PRC05X050PPAHCEL01	PRC Column 5x50 PPA HyperCel	1 mL
PRC08X100MEPHCEL01	PRC Column 8x100 MEP HyperCel	5 mL
PRC08X100HEAHCEL01	PRC Column 8x100 HEA HyperCel	5 mL
PRC08X100PPAHCEL01	PRC Column 8x100 PPA HyperCel	5 mL