PRC Prepacked Chromatography Columns with Q and CM Ceramic HyperD® Ion Exchange Sorbents

Rapid selectivity screening of Ceramic HyperD ion exchangers

• Convenient – Ready-to-use 1 mL prepacked columns.
• Easy to use – Direct connection to commonly-used laboratory chromatography systems such as ÄKTA* systems.
• Efficient – High packing efficiency (> 2,500 plates/meter).
• Consistent – Screen Pall ion exchange chromatography sorbents under reliable and reproducible conditions, and guarantee performance run after run.

Ion Exchange Chromatography Sorbents

Q and CM Ceramic HyperD sorbents have a good capacity hydrogel polymerized within the gigapores of a rigid ceramic bead. Typical features include:

• Good dynamic binding capacity independent of flow rate
• Allowing a direct capture with high binding capacity for IgG at conductivities of 10 to 15 mS/cm on CM Ceramic HyperD sorbent

PRC columns help to determine the best selectivity and resolution in optimal conditions of packing and use.

*ÄKTA is a trademark of GE Healthcare companies.

Rapid screening and condition optimization in the 1 mL PRC column enables rapid selection of the appropriate chemistry.

Two or more columns can be connected in series to increase the column height and more closely model real conditions in pilot scale or for scale down applications. Further scale up can be achieved with minimal re-optimization by packing Pall LRC empty glass columns.

Ion Exchange Chromatography Sorbents

• Native and recombinant proteins
• Plasmids
• Vaccines
• Monoclonal and polyclonal antibodies
• Plasma derivatives
• Biopharmaceuticals

Sorbent Specifications

Volume
1 mL (5 mm ID x 50 mm)

Functionalities
Ion exchange: Q and CM Ceramic HyperD F sorbents

Storage Solution
20% ethanol/150 mM NaCl

Working Pressure¹
< 1.5 barg (22 psig)

Column Specifications

Outer Dimensions
10 x 100 mm for 1 mL columns

Materials of Construction
Body and end caps: Molded polypropylene
17 µm frit: PP/PE (Polypropylene/Polyethylene)

Connections²
Built-in 10-32 fittings

Maximum Operating Pressure
20 barg (290 psig) for 1 mL columns

¹ Pressure at 600 cm/h equivalent to 2 mL/min in 0.1 M NaCl.
² For HPLC/MPLC/AKTA systems, direct connection with 1/16 in. tubing and 10-32 fittings. For connecting to systems with M6 or 1/4-28 fittings, consult the appropriate system manual for necessary fittings and adapters.

Sorbent		Average Particle Size (µm)	Ionizable Groups (µEq/mL)	Dynamic Capacity (mg/mL)1
Ion Exchange	Chemistry
Q Ceramic HyperD F	Quaternary amine	50	> 250	> 852
CM Ceramic HyperD F	Carboxymethyl	50	> 250	> 603

¹ Determined at 10% breakthrough using:
² 5 mg/mL BSA in 50 mM Tris-HCl buffer, pH 8.6, flow rate 200 cm/h
³ 5 mg/mL human IgG in 50 mM sodium acetate buffer, 100 mM NaCl, pH 4.7, flow rate 200 cm/h

Separation of Cytochrome C, BSA and Human Transferrin on a Pall PRC Q Ceramic HyperD F Prepacked Column

Column: Pall PRC Column 5x50 Q Ceramic HyperD F, 5 mm I.D. x 50 mm, volume: 1 mL, linear flow rate: 150 cm/h. Equilibration and wash: 50 mM Tris-HCl, pH 8.6. Load: 100 µL (5 mg/mL cytochrome C, 20 mg/mL BSA and 20 mg/mL human transferrin in equilibration buffer). Elution: 50 mM Tris-HCl, pH 8.6 + 0.5 M NaCl by linear gradient from 0 to 100% in 60 minutes.

Pall offers a comprehensive line of chromatography columns from lab to process scale.

	PRC Columns 1 - 5 mL (prepacked) Fast selectivity screening with Q and S HyperCel Ion Exchange Sorbents with Q and CM Ceramic HyperD Ion Exchange Sorbents with MEP, HEA, and PPA HyperCel Mixed-mode Sorbents
	LRC Columns 5 - 900 mL Preparative and process optimization
	Resolute® Columns 6 - 300 L Pilot and small-scale production
	Resolute Columns 50 - 1500 L Large-scale production