PRC Prepacked Chromatography Columns with Q and S HyperCel™ Ion Exchange Sorbents

Rapid selectivity screening of Q and S HyperCel ion exchangers

• Convenient – Ready-to-use 1 mL and 5 mL prepacked columns.
• Easy to use – Direct connection to commonly-used laboratory chromatography systems such as ÄKTA* systems.
• Efficient – High packing efficiency (> 2,500 plates/meter).
• Consistent – Screen Pall ion exchange chromatography sorbents under reliable and reproducible conditions, and guarantee performance run after run.

Ion Exchange Chromatography Sorbents

Q and S HyperCel sorbents are composed of a rigid cellulose matrix that has excellent flow properties and generates low backpressure. They provide:

• High dynamic binding capacity at short residence time
• Use in either capture or intermediate steps
• Fast re-equilibration, allowing buffer and time savings
• Direct scale up to pilot or production scale columns

*ÄKTA is a trademark of GE Healthcare companies.

Rapid screening and condition optimization in the 1 mL PRC column enables rapid selection of the appropriate chemistry. Once the chemistry is selected on a 1 mL PRC column, the conditions of use can be optimized in a 5 mL PRC column in doubling the height.

Two or more columns can be connected in series to increase the column height and more closely model real conditions in pilot scale or for scale down applications. Further scale up can be achieved with minimal re-optimization by packing Pall LRC empty glass columns.

Ion Exchange Chromatography Sorbents

• Native and recombinant proteins
• Plasmids
• Vaccines
• Monoclonal and polyclonal antibodies
• Plasma derivatives
• Biopharmaceuticals

Sorbent Specifications

Volume
1 mL (5 mm ID x 50 mm)
5 mL (8 mm ID x 100 mm)

Functionalities
Ion exchange: Q and S HyperCel

Storage Solution
30% isopropanol/100 mM sodium phosphate, pH 4.3

Working Pressure¹
< 0.5 barg (7 psig)

Column Specifications

Outer Dimensions
10 x 100 mm for 1 mL columns
11.5 x 140 mm for 5 mL columns

Materials of Construction
Body and end caps: Molded polypropylene
17 µm frit: PP/PE (Polypropylene/Polyethylene)

Connections²
Built-in 10-32 fittings

Maximum Operating Pressure
20 barg (290 psig) for 1 mL columns
30 barg (435 psig) for 5 mL columns

¹ Pressure at 600 cm/h equivalent to 2 mL/min in 0.1 M NaCl.
² For HPLC/MPLC/AKTA systems, direct connection with 1/16 in. tubing and 10-32 fittings. For connecting to systems with M6 or 1/4-28 fittings, consult the appropriate system manual for necessary fittings and adapters.

Sorbent		Average Particle Size (µm)	Ionizable Groups (µEq/mL)	Dynamic Capacity (mg/mL)1
Ion Exchange	Chemisty
Q HyperCel	Quaternary amine	75	99 – 138	> 1602
S HyperCel	Sulfonic acid	75	59 – 84	> 1353

¹Determined at 10% breakthrough using:
² 5 mg/mL BSA in 50 mM Tris-HCl, pH 8.5 at 2 min residence time
³ 5 mg/mL human IgG in 50 mM sodium acetate, pH 4.5 at 2 min residence time

Separation of Model Proteins (Ovalbumin, ß-lactoglobulin, Cytochrome C and Lysozyme) on 1 mL PRC S HyperCel Prepacked Columns and Scale Up on 5 mL Column, Constant Residence Time of 2 Minutes

Columns: Pall S HyperCel PRC prepacked columns of 1 mL (5 mm I.D. x 50 mm) and 5 mL (8 mm I.D. x 100 mm). Load: for 1 mL column: 100 µL ovalbumin (10 mg/mL), ß-lactoglobulin (10 mg/mL), cytochrome C (2.5 mg/mL) and lysozyme (5 mg/mL). For 5 mL column: 500 µL of same proteins. Equilibration: 50 mM Na acetate, pH 4.5. Elution: 50 mM Na acetate, pH 4.5 + 0.5 M NaCl by linear gradient from 0 to 100%.

Pall offers a comprehensive line of chromatography columns from lab to process scale.

	PRC Columns 1 - 5 mL (prepacked) Fast selectivity screening with Q and S HyperCel Ion Exchange Sorbents with Q and CM Ceramic HyperD Ion Exchange Sorbents with MEP, HEA, and PPA HyperCel Mixed-mode Sorbents
	LRC Columns 5 - 900 mL Preparative and process optimization
	Resolute® Columns 6 - 300 L Pilot and small-scale production
	Resolute Columns 50 - 1500 L Large-scale production