Pall Corporation (PLL): Filtration, Separations, Purification & Environmental Technology
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Centrifugal Devices

Pall offers a variety of centrifugal devices for processing many common biological samples. Centrifugal devices, also termed spin filters, spin devices, centrifugal concentrators, or ultrafiltration devices, can be used for a variety of laboratory applications.

Pall's ultrafiltration centrifugal devices simplify many common nucleic acid and protein handling procedures. These devices are offered in a variety of sizes to provide efficient concentration of proteins of nucleic acids. They are also a convenient alternative to dialysis in order to perform buffer exchange or desalting of biomolecules, including proteins, DNA, and viruses. Samples from 50 µL to 60 mL can be processed in just minutes.

Choose from membranes that have been developed to assure low non-specific biomolecule binding and provide consistent, high recovery of target molecules. Ultrafiltration reduces the amount of handling that can cause damage to samples, leaving concentrated samples ready for direct incorporation into downstream applications at critical stages in the discovery process.

Pall's microfiltration centrifugal devices are used in separation and small-scale general filtration procedures. A common application is the clarification of cell culture media in order to capture secreted proteins. These devices can also be used in combination with chromatography resins to create a fast, efficient method for purifying proteins of interest. Devices are available in a range of sizes to accommodate your sample volumes.

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Centrifugal Devices

 
Sample Volume Centrifugal Device
 > 0.5 mL  Nanosep®
 0.5 mL – 5.0 mL  Microsep™ Advance
 5 mL – 20 mL  Macrosep® Advance
 15 mL – 60 mL  Jumbosep™
 

AcroPrep™ Advance filter plates for ultrafiltration can be used with smaller sample volumes (< 100 µL up to 1 mL) in similar applications. 

Pall's Centrifugal Devices Feature:

  • Rapid processing: Achieve high recoveries (typically greater than 90%) in as little as five to ten minutes.  
  • High performance membranes: Omega™ polyethersulfone ultrafiltration membrane provides higher flow rates and is lower protein binding than competitive membranes. This results in lower processing time and the highest possible recoveries.
  • Low protein binding: Devices are constructed of low-binding polypropylene to maximize sample recovery. 
  • Variety of MWCO’s and pore sizes: Available with low-binding microfiltration membranes for particulate removal or chromatography separations. Also available with ultrafiltration membrane for rapid concentrating and/or desalting of proteins.
  • Easy to use: Once you have identified the right MWCO or pore size ranging from 1 kD to 0.45 µm, the devices are color-coded for easy visual identification.
  • Ideal for fast batch mode chromatography applications: Nanosep® centrifugal devices with microfiltration membrane serve as a perfect housing for chromatography resin. The spin device can be filled with the resin of choice to perform the desired protein purification application. 

Typical Applications for Centrifugal Devices

  • Protein concentration or protein fractionation
  • Nucleic acid, DNA, or RNA concentration
  • Desalting or buffer exchange
  • Clean-up of labeling and PCR reactions
  • Removal of free nucleotides
  • Virus concentration or removal
  • Sample prep prior to HPLC
  • Clarification of cell culture media
  • Capture or concentration of secreted proteins

Products

Jumbosep Centrifugal Devices
Convenient and reliable concentration, purification, and diafiltration of 15 to 60 mL biological samples. Jumbosep Centrifugal Devices concentrate sample volumes in 30 minutes and provide high recoveries.  


Macrosep Advance Centrifugal Devices
Macrosep Advance Centrifugal DevicesQuickly concentrates up to 20 mL of biological sample without valuable sample loss




Microsep Advance Centrifugal Devices
Microsep Advance Centrifugal DevicesPrecise, quick recovery of microliter volumes of concentrate from starting volumes up to 5.0 mL



Nanosep and Nanosep MF Centrifugal Devices
Simple, reliable concentrating and desalting of samples, with typical recoveries of greater than 90%. Available with low protein-binding Omega, Bio-Inert®, and GHP membrane.



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