Various methods and technologies exist to purify proteins. The characteristics of the protein of interest such as solubility, charge, size, hydrophobicity, and affinity, along with parameters such as sample type, size, purity, efficacy and abundance of desired protein in sample will help you determine the best chromatography method and media for use. (Screening of methods is easily determined using Acrosep pre-packed chromatography columns or by packing 96-well plates or spin columns.)
In many areas, chromatography resins are the media of choice for chromatography applications, but in some cases where resin-based methods have limitations (for example the purification of viruses or large molecules), membranes have proven to be a robust, scalable and economic alternative. Membranes perform well in certain applications because of their fast flow rates compared to chromatography resins. To meet the needs of such applications, Pall offers Mustang® ion exchange chromatography membranes.