Selection Guide: Ampoule Media and Filters for Microbiological Analysis
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Pall ampoule media is available in a variety of selective, nutrient broths for use in municipal water, food and beverage, pharmaceutical, and microelectronics industries. Sterile ampoule media is premeasured for maximum efficiency and convenience. To find the best microbiological media4 and membrane filters for your application, select your Target Organism (or Method Number2) below. To order, see the product datasheet.
- Total Coliforms (Method Number 9222)
- Fecal Coliforms/E. coli (Method Number 9222)
- Total Bacteria (Method Number 9215; USP 61, 71)
- Lactobacillus sp.
- Yeasts and Molds (Method Number 9610)
- KF Strep Verification (Method Number 9230)
- Pseudomonas sp. (Method Number 9213)
- Total Suspended Solids (Method Number 2540)
- Cryptosporidium or Giardia (U.S. EPA 1622 and 1623, U.K. DWI, ISO/DIS 15553:2006)
Total Coliforms (Method Number 9222)
MF-Endo Broth: E. coli, 24 hour culture at 35 °C
Recommended Media Broth:
| Broth | MF-Endo |
|---|---|
| Target Organism | Total Coliforms |
|
Recovery |
> 85% |
| Test Organism | E. coli |
| pH at 25 °C | 7.2 +/- 0.2 |
| Shelf Life (2 - 8 °C) | 1 year |
| Media Color | pinkish red |
| Target Colony Color | dark red with metallic sheen |
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Fecal Coliforms/E. coli (Method Number 9222)
M-FC Broth: E. coli, 24 hour culture at 44.5 °C
M-FC Broth with rosolic acid: E. coli, 24 hour culture at 44.5 °C
Recommended Media Broth:
| Broth | |
|---|---|
| Target Organism | Fecal Coliforms |
|
Recovery |
> 85% |
| Test Organism | E. coli |
| pH at 25 °C | 7.4 +/- 0.2 |
| Shelf Life (2 - 8 °C) | 1 year |
| Media Color | blue/violet |
| Target Colony Color | blue |
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Total Bacteria (Method Number 9215; USP 61, 71)
M-TGE Broth: S. epidermidis, 24 hour culture at 35 °C
Tryptic Soy Broth USP: S. epidermidis, 24 hour culture at 35 °C
HPC Broth: S. epidermidis, 24 hour culture at 35 °C
Recommended Media Broth:
| Broth | M-TGE | Trypticase Soy USP |
HPC with HPC indicator |
|---|---|---|---|
| Target Organisms | Total Bacteria | Total Bacteria | Total Bacteria |
| Recovery (versus control) |
> 85% | > 85% | > 85% |
| Positive Control Test Organism(s) |
E. coli; S. epidermidis |
E. coli | E. coli; S. epidermidis |
| pH at 25 °C | 7.0 + 0.2 | 7.0 + 0.2 | 7.3 + 0.2 |
| Shelf Life (2 - 8 °C) |
1 year* | 1 year | 1 year |
| Media Color | pale yellow | pale yellow | pale yellow |
| Colony Color | organism dependent |
red | organism dependent |
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Lactobacillus sp.
Orange Serum Broth: L. plantarum, 72 hour culture at 35 °C
Recommended Media Broth:
| Broth | Orange Serum |
|---|---|
| Target Organism | Lactobacillus, Acid Resistant Bacteria |
|
Recovery |
> 85% |
| Test Organism | L. plantarum; S. cerevisiae |
| pH at 25 °C | 5.6 +/- 0.2 |
| Shelf Life (2 - 8 °C) | 1 year |
| Media Color | dark amber |
| Target Colony Color | organism dependent |
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Yeasts and Molds (Method Number 9610)
M-Green YM Broth: S. cerevisiae, 24 hour culture at 35 °C
Recommended Media Broth:
| Broth | |
|---|---|
| Target Organism | Yeasts and Molds |
|
Recovery |
> 85% |
| Test Organism | S. cerevisiae |
| pH at 25 °C | 4.6 +/- 0.2 |
| Shelf Life (2 - 8 °C) | 1 year* |
| Media Color | green |
| Target Colony Color | pale green |
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KF Strep Verification (Method Number 9230)
KF-Streptococcal Broth: S. faecalis, 48 hour culture at 35 °C
Recommended Media Broth:
| Broth | |
|---|---|
| Target Organism | Fecal Streptococcus |
|
Recovery |
> 85% |
| Test Organism | S. faecalis |
| pH at 25 °C | 7.2 +/- 0.2 |
| Shelf Life (2 - 8 °C) | 1 year |
| Media Color | light purple |
| Target Colony Color | red |
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Pseudomonas sp. (Method Number 9213)
Pseudomonas Broth: P. aeruginosa, 48 hour culture at 35 °C
Recommended Media Broth:
| Broth | |
|---|---|
| Target Organism | Pseudomonas sp. |
|
Recovery |
> 85% |
| Test Organism | Ps. aeruginosa |
| pH at 25 °C | 7.1 +/- 0.2 |
| Shelf Life (2 - 8 °C) | 1 year |
| Media Color | light amber |
| Target Colony Color | green-blue |
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Cryptosporidium or Giardia (U.S. EPA 1622 and 1623, U.K. DWI, ISO/DIS 15553:2006)
Recommended filters:
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