Life Sciences Laboratory Literature Library

Affinity Chromatography Selection Guide (Redirects to 52126)

Affinity chromatography separates compounds based on the properties of chosen ligands with specific affinity to the molecule being selected for attachment to the solid matrices. Often, the binding between the molecule and the ligand can be reversed after the selection step. This is done, for example, through buffer, salt concentration, or pH change. Pall offers bottled resins and packaged kits for affinity chromatography. Pall bottled resins can be used for small and large sample sizes, and for single use and high throughput methods of purification. Our base resin varies depending on targeted application.


Type of Affinity Purification	Ligand	Pall Product
Albumin Depletion	Blue Dye	Enchant™ Multi-Protein Affinity Separation Kit Blue Trisacryl® M Chromatography Resin
IgG Purification	Protein A Protein G	Enchant Multi-Protein Affinity Separation Kit Protein A Ceramic HyperD® Chromatography Resin MEP HyperCel™ Chromatography Resin
Lipoprotein Purification	Heparin	Heparin HyperD M Chromatography Resin
Glycoprotein Purification	Lysine	Lysine HyperD Chromatography Resin HA Ultrogel® Chromatography Resin

Resins for Affinity Chromatography

Protein A Ceramic HyperD® F Resin is a high capacity affinity resin designed for process-scale purification of immunoglobulin G. The resin combines ease of use with high binding capacity and excellent scalability. Protein A Ceramic HyperD F resin is prepared using a rigid proprietary ceramic bead. Recombinant Protein A is immobilized to a specially formulated hydrogel within the porous ceramic bead.

Blue Trisacryl® M Resin is an affinity chromatography resin used for the purification of a wide variety of enzymes and proteins such as kinases, albumin, interferons, and some coagulation factors. The basic matrix is Trisacryl GF2000, a macroporous non-ionic resin on which Cibacrons Blue is covalently immobilized. Cibacron Blue F3GA dye is strongly bound to the matrix through a six carbon spacer arm. The reaction is performed with the EEDQ coupling agent. This type of coupling prevents any leakage of the dye in normal working conditions.

Heparin HyperD M Resin is a high speed, high capacity affinity preparative resin for the purification of biological molecules that bind to heparin, such as coagulation factors, growth factors, lipoproteins, etc. The resin provides high binding capacity at high flow rates. Heparin HyperD M affinity resin employs a high-capacity hydrogel polymerized within the large pores of a rigid bead. This design combines the desirable characteristics of a soft, high capacity hydrogel with the high dimensional stability of a rigid bead.

Lysine HyperD Resin is a high speed, high capacity affinity preparative resin for the purification of biological molecules that bind to lysine such as plasminogen from human or animal species plasma. The resin provides high binding capacity at high flow rates. Lysine HyperD affinity resin employs a high-capacity hydrogel polymerized within the large pores of a rigid bead. This design combines the desirable characteristics of a soft, high capacity hydrogel with the high dimensional stability of a rigid bead.

Enchant™ Protein Purification Kits rapidly deplete unwanted abundant proteins and unmask low abundant biomarkers from human and animal-derived serum and plasma samples. Alternatively, these same kits can be used to collect the abundant protein fraction for further analysis. Convenient kit formats eliminate the need to handle messy slurries or deal with column packing. These all-in-one kits include the protocol, purification columns and buffers, and offer one of the lowest costs per sample available.

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Resins for Metal-chelate Affinity Chromatography

Metal-chelate affinity chromatography exploits the affinity of proteins or other molecules for metal ions. The affinity is derived from the formation of coordination bonds between the metal ions and certain exposed side chains of protein amino acids. Histidine, present in many proteins, forms complexes with transition metal ions such as Cu^–, Zn²⁺, Ni²⁺ or Fe³⁺. IMAC HyperCel™ Resin uses tridentate IDA (iminodiacetic acid) as a chelating ligand agent. This ligand is immobilized on the HyperCel base resin, a stable, robust and well-known resin used for both research and industrial-scale protein separations.

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