Pall Corporation (PLL): Filtration, Separations, Purification & Environmental Technology
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Protein Fractionation

Protein Fractionation Sections

Protein Fractionation Introduction

Sample complexity reduction is an important first step to facilitating access to the low abundant proteins of interest for clinical research and diagnostics. The process for human serum and plasma frequently includes the depletion of highly abundant proteins such as albumin and IgG in combination with other fractionation technologies, such as ion exchange or affinity separation, prior to 2D gel or LC-MS/MS analyses. 

Pall currently offers several solutions for small-scale chromatographic protein fractionation (see Table 2.8). Options include chromatography resin for affinity capture, ion exchange separation, and mixed-mode capture resins. Pall also offers membrane chromatography solutions, i.e., Mustang® membranes. All chemistries are available for use in several forms including standard chromatography columns, disposable columns, spin filters, and multi-well filter plates.		  















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Table 2.8

Protein Fractionation Quick Selection Guide
 
Media Description Formats Available Separation Mechanism
Ion Exchange 
Q Ceramic HyperD F Resin Bottled resin Strong anionic exchanger;
 binds negatively charged target
Mustang Q Membrane unit Syringe column,
 multi-well filter plate		 Strong anionic exchanger;
 binds negatively charged target
S Ceramic HyperD F Resin Bottled resin Strong cationic exchanger;
 binds positively charged target
Mustang S Membrane unit Syringe column,
 multi-well filter plate		 Strong cationic exchanger;
 binds positively charged target
DEAE Ceramic HyperD F Resin Bottled resin Weak anion exhanger
CM Ceramic HyperD F Resin Bottled resin Weak cationic exchanger
Affinity
Heparin Resin Bottled resin Direct binding to targets with
 an affinity for Heparin
Lysine Resin Bottled resin Direct binding to targets with
 an affinity for Lysine
IMAC Resin Bottled resin Binds tagged proteins using
an immobilized metal compound
Mixed Mode
MEP Resin  Bottled resin Uses several binding
 mechanisms including
 hydrophobic interactions
HA Ultrogel® Resin Bottled resin Mixed mode
Size Exclusion
Ultrogel Resin Bottled resin Separates targets by size
 

Table 2.9

Available Separation Columns   

 
Description Column Volume Available from Pall
 Glass Chromatography Column Varies No
 Disposable Chromatography Column Varies No
Spin Filter < 1 mL Yes
Deep Well Multi-Well Filter Plate 96 x < 1 mL Yes
Multi-Well Filter Plate 96 x < 350 µL Yes
 

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