Protein Concentration, Protein Desalting, and Buffer Exchange
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Concentration, Desalting, and Buffer Exchange Sections
Concentration, Desalting, and Buffer Exchange IntroductionBiomolecule purification involves a complex series of steps where targets are selectively separated through sequential processes. The processes by which separation is performed often creates a need for the sample to be desalted or concentrated to prepare the biomolecule sample for the next step in the purification process. Pall offers several technologies to perform efficient sample concentration, desalting, and buffer exchange including ultrafiltration (UF) spin filters, UF multi-well filtration plates, and bottled gel filtration media. Refer to Table 2.38 for a selection of UF products available. Compared to other methods, UF methods for concentration and desalting offer a number of advantages including:
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Table 2.38
UF Products for Concentration and Desalting
Table 2.39
Properties of Ultrogel® AcA and Trisacryl® GF-05 Resin
Figure 2.34
Centrifugal Spin Filters
UF Products for Concentration and Desalting
| Device | Sample Volume |
| AcroPrep™ 384 filter plate | < 100 μL |
| AcroPrep 96 filter plate | < 350 μL |
| Nanosep® device | < 0.5 mL |
| Microsep™ device | 0.5-3.5 mL |
| Macrosep® device | 3-15 mL |
| Jumbosep™ device | 15-60 mL |
Table 2.39
Properties of Ultrogel® AcA and Trisacryl® GF-05 Resin
| Specification | Ultrogel AcA 202 Resin | Trisacryl GF-05M Resin |
| Particle Size | 60-140 μm | 40-80 μm |
| Monomer | 20% (w/v) Acrylamide | N-acryloyl-2-amino-2- hydroxymethyl-1,3-propanediol |
| Cross-linker | 2% (w/v) Agarose | Hydroxylated acrylic bifunctional monomer |
| Exclusion Limit | 22,000 dalton | 3,000 dalton |
| Linear Fractionation Range | 1,000-15,000 dalton | 200-2,500 dalton |
| Resolving Power (plates/m) | 3,000 | 2,500 |
| Working pH Range | 3-10 | 1-11 |
Figure 2.34
Centrifugal Spin Filters