Chromatography Resins for Protein Purification
Print Product Information
E-Mail
Download PDF
BioSepra® Chromatography Resin Sections
Chromatography Resins IntroductionChromatography continues to be an essential technology for the purification of biomolecules. Pall offers a line of chromatography resins ideal for protein purification applications (see Table 4.6). This broad line of chromatography products exhibits superior performance and is useful for affinity, ion exchange, size exclusion, and hydrophobic interaction chromatography (HIC). Unique mixed-mode BioSepra products also exist to provide solutions to current sample preparation challenges. The resins Pall offers for small-scale applications are the same ones offered to our customers currently manufacturing biopharmaceuticals. The ability to scale up is essential for those working in drug discovery, development, and manufacturing. These resins can be used in varying size chromatography columns, as well as in batch mode for single or high throughput mode. This is ideal for quick preps or in situations where optimizing purification conditions is required. Table 4.6Chromatography Resins |
              |
| Chromatography Type | Product Name | Description |
| Ion Exchange | Q Ceramic HyperD® | Strong anionic exchanger, binds negatively-charged target |
| S Ceramic HyperD | Strong cationic exchanger, binds positively-charged target | |
| CM Ceramic HyperD | Weak cationic exchanger | |
| DEAE Ceramic HyperD | Weak anionic exchanger | |
| Affinity | Protein A Ceramic HyperD | Binds IgG |
| Blue Trisacryl® | Binds albumin | |
| Heparin HyperD | Direct binding to targets that have an affinity for heparin | |
| Lysine HyperD | Direct binding to targets that have an affinity for lysine | |
| IMAC HyperCel™ | Binds tagged proteins using an immobilized metal compound | |
| SDR HyperD | Detergent removal | |
| Mixed Mode | MEP HyperCel | Uses several binding mechanisms including hydrophobic interactions |
| HA Ultrogel® | Hydroxyapatite | |
| HCIC | MEP HyperCel | Uses several binding mechanisms including hydrophobic interactions |
| Size Exclusion | Ultrogel AcA | Separates targets by size |
Table 4.7
Available Separation Columns| Description | Column Value | Available from Pall |
| Glass Chromatography Column | Varies | No |
| Disposable Chromatography Column | Varies | No |
| Spin Filter | <1 mL | Yes |
| Deep Well Multi-Well Filter Plate | 96 x <1 mL | Yes |
| Multi-Well Filter Plate | 96 x <350 µL | Yes |