Process Proteomics

Process Proteomics Sections Introduction  Screening of a Resin in a Multi-Well Filter Format Column Scale-Up to Pilot Process Applications Process Proteomics Introduction Chromatography continues to be an essential technology for the purification of biomolecules. Pall offers a line of chromatography resins ideal for protein purification applications. This broad line of chromatography products exhibits superior performance and is useful for affinity, ion exchange, size exclusion, and hydrophobic interaction chromatography (HIC). Unique mixed-mode BioSepra® products also exist to provide solutions to current sample preparation challenges.  The resins Pall offers for small-scale applications are the same ones offered to our customers currently manufacturing biopharmaceuticals. The ability to scale up is essential for those working in drug discovery, development, and manufacturing. These resins can be used in varying size chromatography columns, as well as in batch mode for single or high throughput mode. This is ideal for quick preps or in situations where optimizing purification conditions is required.  Pall also offers recommendations and services for streamlining initial scouting of protein purification conditions through our Process Proteomic Service Centers. The identification and optimization of protein purification parameters can be a tedious task. Process proteomic methodologies can be used to streamline the initial scouting of protein purification conditions, as well as for some of the optimization steps. Process proteomics is performed using common chromatographic chemistries (e.g., anion exchange, cation exchange, IMAC) on a protein chip, in the wells of

a multi-well filter plate, or using small columns. Using this approach, multiple binding, washing, and elution conditions can be tested on your sample simultaneously. Successful scale-up from these small-scale experiments to traditional chromatography columns has proven to be quite useful.