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Chromatography Products Selection Guide Appendix 6.5

Proteomics, Protein Chemistry, Protein Purification   Proteomics Products   Proteomics Product Selection Guide

Complete Protein Application Manual

Click to expand/collapse this sectionProteomics Manual: Table of Contents

Chromatography Products Selection Guide 

 

Chromatography
Type

Product

Description		 Particle Size (Average)

Capacity
Primary
Applications
Size Exclusion (Gel Filtration)
Separation by Molecule Size Bulk Resin
Ultrogel® AcA Ultrogel AcA are  polymeric resins for size exclusion composed of polyacrylamide and agarose, characterized by narrow particle size distribution.   100 μm N/A Fractionation, purification of biomolecules by size, molecular weight
Ion Exchange
Separation by Charge Bulk Resin
Q Ceramic HyperD® F Strong cation exchanger Ceramic HyperD ion exchangers employ a high capacity hydrogel polymerized within the large pores of a rigid ceramic bead. 50 µm >85 mg/mL4 Recombinant proteins, monoclonal antibodies, plasmid, vaccine purification. capture step
S Ceramic HyperD F Strong cation exchanger Ceramic HyperD ion exchangers employ a high capacity hydrogel polymerized within the large pores of a rigid ceramic bead. 50 µm >75 mg/mL5 Recombinant proteins, monoclonal antibodies, plasmid, vaccine purification. capture step
DEAE Ceramic HyperD F Weak cation exchanger Ceramic HyperD ion exchangers employ a high capacity hydrogel polymerized within the large pores of a rigid ceramic bead. 50 µm >85 mg/mL4 Recombinant proteins, monoclonal antibodies, plasmid, vaccine purification. capture step
CM Ceramic HyperD F Weak cation exchanger Ceramic HyperD ion exchangers employ a high capacity hydrogel polymerized within the large pores of a rigid ceramic bead. 50 µm >60 mg/mL6 Recombinant proteins, monoclonal antibodies, plasmid, vaccine purification capture step
Membrane Filter Plates and Devices
Mustang Q Strong anion exchanger. Also available in AcroPrep® 96 filter plates 350 µL or 1 mL, and Acrodisc syringe filters N/A 50-60 mg/mL Protein fractionation
Mustang S Strong anion exchanger. Also available in AcroPrep® 96 filter plates 350 µL or 1 mL, and Acrodisc syringe filters N/A 45-50 mg/mL Protein fractionation
Affinity
Separation Using Specific Ligands
Bulk Resin
Blue Trisacryl® M Blue Trisacryl  M is an affinity chromatographic resin used for the purification of a wide variety of enzymes and proteins such as kinases, albumin, interferons, and some coagulation factors. The basic matrix is Trisacryl GF2000, a macroporous, nonionic resin on which Cibacron* blue is covalently immobilized. 60 µm HSA: 10-15 mg/mL BSA: 5-7 mg/mL1 Albumin depletion
IMAC HyperCel™ IMAC HyperCel™ uses tridentate IDA(imino- diacetic-acid) as a chelating agent. The ligand is immobilized on the HyperCel base sorbent, a stable and robust resin. 90 μm 30-60 µmol Cu++/mL resin Tagged biomolecule purification
Protein A Ceramic HyperD® F Protein A Ceramic HyperD F is a high capacity affinity resin prepared using a rigid proprietary ceramic bead. Recombinant Protein A is immobilized to a specially formulated hydrogel within the porous ceramic bead. 50 µm >30 mg/mL2 IgG purification/ depletion
Heparin HyperD M Heparin HyperD M composite chromatography resin is used to purify biological molecules that bind to heparin such as coagulation factors, growth factors, and lipoproteins. Heparin HyperD M is composed of a porous rigid mineral bead containing heparin bound hydrogel filled pores. 80 µm >30 mg/mL2 Purification of coagulation factors, lipoproteins, growth factors, nucleic acid binding enzymes.
Lysine HyperD Lysine HyperD is used to purify biological molecules that bind to lysine such as glycoproteins. Lysine HyperD is comprised of a porous rigid mineral bead containing lysine (L-lysine) bound hydrogel filled pores. 70 µm N/A Purification of glycoproteins
Mixed Mode and Hydrophobic Charge Induction (HCIC)
A Unique Combination of Chromatography Modes


Bulk Resin
MEP HyperCel™ MEP HyperCel (4-mercapto-ethyl-pyridine) resin is specifically designed for the capture and purification of monoclonal and polyclonal antibodies. In contrast to Protein A resins, IgG binding on MEP HyperCel is essentially independent of subclass or species. Weakly binding variants (e.g., murine IgG, rat IgG) are well retained/ 90 µm >20 mg/mL7 Purification/ depletion of polyclonal and monoclonal antibodies of most species.
SDR HyperD® SDR HyperD is a mixed mode of size exclusion, normal phase, and a reversed phase. It is a unique resin designed to eliminate solvent and detergent while recovering NATIVE protein.
SDR HyperD is a composite resin that combines a silica bead moiety filled with long chain aliphatic polymers that are cross-linked to provide a 3D mesh with a low size exclusion limit of 10 kDa which excludes proteins.		 80 µm 60-80 mg/mL13 Solvent and detergent removal
Hydroxyapatite
Protein Interaction with Calcium Phosphate
Bulk Resin
_________  _________________  _______  ___________  ________________
HA Ultrogel® HA Ultrogel hydroxyapatite resin is composed of cross-linked agarose beads with micro-crystals of hydroxyapatite entrapped in the agarose mesh 120 µm Cytochrome C: >7 mg/mL9 Immunoglobulin separation, glycoproteins, vaccines
 

Footnotes — Chromatography Products Selection Guide

  1. Capacity determined in PBS buffer using 5 mg/mL.
  2. Dynamic binding capacity, 10% breakthrough, 100 cm/h, determined using 10 mg/mL hu IgG in PBS, pH 7.4; elution in 0.1 M sodium citrate, pH 2.5; column 4.6 ID x 100 mm.
  3. Dynamic binding capacity at 600 cm/h, using hu ATIII at 72.5 UI/mL in 20 mM Tris-HCl, 0.3 M NaCl, pH 7.4; elution with 20 mM Tris-HCl, 2 M NaCl, pH 7.4; 10 cm bed height.
  4. Dynamic binding capacity, 10% breakthrough, 200 cm/h; sample: 5 mg/mL BSA in 50 mM Tris-HCl buffer, pH 8.6.
  5. Dynamic binding capacity, 10% breakthrough, 200 cm/h; sample: 5 mg/mL lysosome in 50 mM sodium acetate, pH 4.5. 
  6. Dynamic binding capacity, 10% breakthrough, 200 cm/h; sample: 5 mg/mL hu IgG in 50 mM sodium acetate, 100 mM NaCl, pH 7.4.
  7. Dynamic binding capacity, 10% breakthrough, determined using 5 mg/mL hu IgG in PBS, flow rate: 60 cm/h.
  8. Dynamic binding capacity, 10% breakthrough, determined using 5 mg/mL hu polyclonal IgG; adsorbtion 50 mM sodium acetate, 0.14 M NaCl, pH 5.5; 5 min residence time.
  9. Capacity for cytochrome c, determined using 5 mg/mL cytochrome c diluted 50/50 in 1 mM phosphate buffer, pH 6.8; at 12.5 cm/h.
  10. Dynamic binding capacity, 10% breakthrough determined using 5 mg/mL BSA in 50 mM Tris-HCl buffer, pH 8.6; 150 mM NaCl.
  11. Dynamic binding capacity, 10% breakthrough, determined using 5 mg/mL hu IgG in 50 mM sodium acetate buffer, pH 4.7; 150 mM NaCl.
  12. Dynamic binding capacity, 10% breakthrough at 300 cm/h, determined using 5 mg/mL Triton* in PBS, pH 7.4. www

Proteomics, Protein Chemistry, Protein Purification   Proteomics Products   Proteomics Product Selection Guide