Clarification of Plasma or Serum Samples to Remove Cryoprecipitate Using Acrodisc® Syringe Filters with a Prefilter 2.5.6

Table 2.62

Properties of the Serum Acrodisc MF Syringe Filter, 32 mm Device 

Specification	Parameter
Materials of Construction      Membrane Supor PES       Prefilter	Hydrophilic polyethersulfone (PES) Binder free borosilicate glass fiber
Device	ABS plastic
Effective Filtration Area	5.8 cm2
PES Membrane Pore Size   Glass Fiber Prefilter Pore Size	0.2 μm 1 μm (nominal) binder-free borosilicate glass
Hold-up Volume	<0.1 mL
Maximum Temperature	55 ºC
Maximum Pressure Limit	5.2 bar (520 kPa, 75 psi)
Typical Water Flow Rate   [mL/min at 3.1 bar (310 kPa, 45 psi)]	425
Inlet/Outlet Connectors	Female luer-lok inlet, male slip luer outlet
Endotoxin Test	< 0.25 EU/mL using Limulus Amoebocyte Lysate (LAL) test
Sterilization	Sterilized by gamma irradiation and individually packaged

Protocol for Clarification of Plasma or Serum Samples to Remove Cryoprecipitate Using Acrodisc® Syringe Filters with a Prefilter

A. Materials Required

1. Serum Acrodisc syringe filter (PN 4525) with GF plus MF membranes. For specifications, see Table 2.62.
2. Empty syringes (10-30 mL volume)

B. Serum Acrodisc Filtration in Syringe Adapted Format

1. Before filling the syringe with sample, draw approximately 1 mL of air into the syringe. This will allow the air to follow the sample out of the syringe. This “air purge” minimizes fluid retention within the filter device.
2. Fill the syringe with the solution to be filtered.
   Tip: Use of syringes smaller than 10 mL can generate excessive pressure on the filter, which may exceed maximum operating pressure.
3. Holding the filter device in one hand and the filled syringe in the other, secure (without excessive force) the filled syringe to the filter device with a twisting motion.
4. Apply gentle pressure to begin filtration. A gentle pressure helps assure maximum throughput.
   Tip: As the filter removes particulate, filtration will become more difficult. The syringe plunger will be harder to push. Pressure will rapidly increase on the filter. Change filters when resistance becomes excessive. Failure to change filter may result in housing rupture, which results in particulate contaminating the filtrate.

Tip: These filters are for SINGLE USE ONLY.

Application Data for Clarification of Plasma or Serum Samples to Remove Cryoprecipitate Using Acrodisc Syringe Filters with a Prefilter

The PES 0.2 μm membrane shows high throughput for filtration of high viscosity samples, such as Dextran, Trypticase Soy Broth, and BSA. The addition of a 0.8 μm PES prefilter to a 0.2 μm PES sterilization membrane considerably improves throughput with viscous, particulate-laden or high protein content solutions, such as serum or plasma. A comparison of filtration of bovine serum or a bacterial culture with a 0.8/0.2 μm Supor® PES membrane Acrodisc filter is summarized in Figure 2.59. The prefilter considerably extends the filtration of these samples beyond 10-20 seconds when the 0.2 μm PES filter reached a plateau of throughput. When a glass fiber prefilter is used, improvement in filtration throughput occurs.

Figure 2.59

Increased Serum Filtration Throughput with a 0.8/0.2 μm Supor® PES Filter Compared to a Single 0.2 μm Membrane

Acrodisc® 0.2 μm (PN 4612) and Acrodisc PF 0.8/0.2 μm (PN 4187) syringe filters with Supor membrane were challenged with calf serum at a constant pressure of 1.4 bar (140 kPa, 20 psi).

Ordering Information for Clarification of Plasma or Serum Samples to Remove Cryoprecipitate

Acrodisc Syringe Filters, Supor Membrane

Part Number	Description	Pkg
4525	Serum Acrodisc syringe filter, GF/0.2 μm, 37 mm, sterile	20/pkg
4187	Acrodisc PF syringe filter, 0.8/0.2 μm, 25 mm, sterile	50/pkg
4658	Acrodisc PF syringe filter, 0.8/0.2 μm, 32 mm, sterile	50/pkg

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