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Prefiltration of Plasma or Serum Samples Using Acrodisc® Syringe Filters with Glass Fiber 2.5.7

Particulate Removal Sections

Prefiltration of Plasma or Serum Samples Using Acrodisc® Syringe Filters with Glass Fiber

Plasma or serum when stored frozen can yield variable amounts of aggregated material on thawing. This aggregated material, if not removed, will rapidly block a microfiltration (MF) filter. As an alternative to centrifugation, this material can be removed by prefiltration using glass fiber. This process is usually used in conjunction with Serum Acrodisc MF final filtration or Acrodisc PF series 0.8/0.2 and 1.2/0.45 μm combination Supor® PES membrane filtration. It is important to employ a final MF filtration step to trap any glass microfibers that may be shed from the Acrodisc prefilter media. 

The Glass Acrodisc syringe prefilter is available with single layer glass fiber or with a multilayered glass fiber prefilter over glass fiber media. The GxF multi-layered prefilter provides two to four times the throughput of standard glass fiber prefilter devices, allowing for quick and easy filtration of your most difficult-to-filter samples, reducing clogging in subsequent MF filtration steps. It is used alone or in series with a final membrane filter to increase flow rate and throughput. For extremely heavy particulate loads, a larger 37 mm size is available. 		 















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Protocol for Prefiltration of Plasma or Serum Samples Using Acrodisc Syringe Filters with Glass Fiber

A. Materials Required

  1. Acrodisc PSF GxF syringe filter (PN AP-4523). For specifications, see Table 2.63.
  2. Empty syringes (10-30 mL volume)  

Table 2.63

Properties of the Acrodisc PSF GxF Syringe Filter, 25 mm

Specification Parameter
 Membrane Prefilter Binder free borosilicate glass fiber
Device Polypropylene
Effective Filtration Area 3.9 cm2
GxF Prefilter Pore Size 1-40 μm (depth filter)
Hold-up Volumes (with Air Purge) < 0.125 mL
Maximum Temperature 82 °C
Maximum Pressure Limit 4.1 bar (410 kPa, 60 psi) at 21-24 ºC
Typical Water Flow Rate (mL/min)
 at 1.0 bar (100 kPa, 15 psi)		 650
Inlet/Outlet Connectors Female luer-lok inlet, male slip luer outlet

B. Prefiltration with an Acrodisc® GxF Membrane

  1. Before filling the syringe with sample, draw approximately 1 mL of air into the syringe. This will allow the air to follow the sample out of the syringe. This “air purge” minimizes fluid retention within the filter device.
  2. Fill the syringe with the solution to be filtered.
    CAUTION: Use of syringes smaller than 10 mL can generate excessive pressure on the filter, which may exceed maximum operating pressure.
  3. Holding the filter device in one hand and the filled syringe in the other, secure (without excessive force) the filled syringe to the filter device with a twisting motion.
  4. Apply gentle pressure to begin filtration. A gentle pressure helps assure maximum throughput.
    Tip: As the filter removes particulate, filtration will become more difficult. The syringe plunger will be harder to use. Pressure will rapidly increase on the filter. Change filters when resistance becomes excessive. Failure to change filter may result in housing rupture, which results in particulate contaminating the filtrate.

Tip: These are for SINGLE USE ONLY.

Ordering Information for Prefiltration of Plasma or Serum Samples Using Acrodisc Syringe Filters with Glass Fiber


Acrodisc PSF GxF Syringe Filters, Glass Fiber

 Part Number Description Pkg
AP-4523 GxF/Glass, 25 mm 50/pkg, 200/cs
AP-4529 GxF/Glass, 25 mm 1000/pkg

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