Jumbosep™ Centrifugal Devices

Convenient and Reliable Concentration, Purification, and Diafiltration of 20 to 60 mL Biological Samples

• Typically concentrates 60 mL sample volumes to 5 mL in 30 minutes
• Provides high recoveries, typically > 90%
• Low protein-binding Omega™ membrane and polysulfone housing minimize losses due to non-specific binding
• Versatile Omega membrane is available in a variety of MWCOs, color-coded for easy identification
• Built-in deadstop prevents spinning to dryness
• Unique sealing mechanism prevents retentate leakage and filtrate contamination
• Economical - Sample reservoir and filtrate receiver can be sanitized or autoclaved, and reused

Replaces dialysis, chemical precipitation, and lyophilization in the following applications:

• Concentrating and desalting proteins
• Exchanging buffer or removing salt from chromatography eluates and gradient fractions
• Separating biomolecules from cell culture supernatants
• Concentrating or removing viruses
• Performing crude fractionation of protein mixtures
• Removing debris and particulates from cell lysates

Materials of Construction

• Filter Media: Omega (modified polyethersulfone) membrane
• Sample Reservoir and Filtrate Receiver: Polysulfone
• Sample Reservoir Cap: Polyethylene
• Insert Without Membrane: High density polyethylene
• Filtrate Receiver Cap and Insert Release: Polypropylene

Effective Filtration Area

• 15.2 cm²

Dimensions

• Outside Diameter (Maximum): 6 cm (2.4 in.)
• Overall Height (Fully Assembled with Cap): 11.3 cm (4.5 in.)

Capacities

• Maximum Sample Volume: 60 mL
• Final Concentrate Volume: 3.5 - 4 mL
• Maximum Filtrate Receiver Volume: 60 mL
• Hold-up Volume (membrane/support): 0.2 mL

Operating Temperature Range

• 0 - 40 °C (32 - 104 °F)

pH Range

• 1 - 14

Centrifuge

• Swinging bucket rotor is required that accepts flat-bottomed 250 mL bottles and is capable of spinning at up to 3,000 x g

Sanitization

• Provided non-sterile. The entire device, including the filter media, may be sanitized by filtering 70% ethanol through it prior to use. The sample reservoir and the filtrate receiver can be autoclaved. Do not autoclave the filter media.

Jumbosep™ Device Reduces Processing Time Over Other Devices

1 mg/mL BSA solution was processed in each of the above devices until a 15-fold concentration is achieved.

 

Concentration Time

Concentrate dilute protein samples in less than 30 minutes with 10, 30, and 100K Jumbosep devices.

• BioTrace™ NT Nitrocellulose Transfer Membrane
• BioTrace™ PVDF Transfer Membrane
• Biodyne® Transfer Membranes
• Macrosep® Advance Centrifugal Devices
• Microsep™ Advance Centrifugal Devices
• Nanosep® & Nanosep MF Centrifugal Devices

Part Number	Description	Pkg	Price	Qty
Jumbosep Centrifugal Device Starter Kits
FD000K65	Generic starter kit, (no membrane inserts)	4/pkg	Call
FD003K65	3K starter kit, gray	4/pkg	Call
FD010K65	10K starter kit, blue	4/pkg	Call
FD030K65	30K starter kit, red	4/pkg	Call
FD100K65	100K starter kit, clear	4/pkg	Call
FD300K65	300K starter kit, orange	4/pkg	Call
Jumbosep Centrifugal Device Membrane Inserts
OD003C65	3K membrane insert, gray	12/pkg	Call
OD010C65	10K membrane insert, blue	12/pkg	Call
OD030C65	30K membrane insert, red	12/pkg	Call
OD100C65	100K membrane inser, clear	12/pkg	Call
OD300C65	300K membrane insert, orange	12/pkg	Call
Accessory Products
FD001X65	Filtrate receiver and cap	12/pkg	Call
FD002X65	Sample reservoir and cap	12/pkg	Call
FD003X65	Insert release	24/pkg	Call