Plasmid Purification and Gene Therapy



Gene therapy is related to vaccines, although the products are mostly intended for therapeutic use, and involves delivering DNA to the target cells in the body, allowing them to produce molecules the lack of which are implicated in genetic disorders, supplement immunity and many other potential treatments.

Production of correctly coded DNA (or RNA) strands for this purpose for use directly in patients, for incorporation into vectors, or into recombinant cells used to produce proteins that are the target product may be challenging, as the DNA molecules are large, strongly charged and often start off in high particulate, viscous solutions.

 








Plasmid Purification and Gene Therapy


Pall has developed numerous products that facilitate purification of DNA entities, and the separation of wanted vs unwanted DNA even of the same molecular weight – for example coiled, supercoiled, nicked plasmids. Mustang® chromatography devices are widely used.

Because of the large number of different vaccines and potential production routes, not every eventuality is covered in these pages. To discuss your specific vaccine application, please contact Pall.