Rapid screening and condition optimization in the 1 mL PRC column enables rapid selection of the appropriate chemistry. Once the chemistry is selected on a 1 mL PRC column, the conditions of use can be optimized in a 5 mL PRC column in doubling the height.
Two or more columns can be connected in series to increase the column height and more closely model real conditions in pilot scale or for scale down applications. Further scale up can be achieved with minimal re-optimization by packing Pall LRC empty glass columns.
Volume 1 mL (5 mm ID x 50 mm) 5 mL (8 mm ID x 100 mm)
Functionalities Ion exchange: Q and S HyperCel
Storage Solution 30% isopropanol/100 mM sodium phosphate, pH 4.3
Working Pressure1 < 0.5 barg (7 psig)
Outer Dimensions 10 x 100 mm for 1 mL columns 11.5 x 140 mm for 5 mL columns
Materials of Construction Body and end caps: Molded polypropylene 17 µm frit: PP/PE (Polypropylene/Polyethylene)
Connections2 Built-in 10-32 fittings
Maximum Operating Pressure 20 barg (290 psig) for 1 mL columns 30 barg (435 psig) for 5 mL columns
1 Pressure at 600 cm/h equivalent to 2 mL/min in 0.1 M NaCl. 2 For HPLC/MPLC/AKTA systems, direct connection with 1/16 in. tubing and 10-32 fittings. For connecting to systems with M6 or 1/4-28 fittings, consult the appropriate system manual for necessary fittings and adapters.
Average Particle Size (µm)
Ionizable Groups (µEq/mL)
Dynamic Capacity (mg/mL)1
99 – 138
59 – 84
1Determined at 10% breakthrough using: 2 5 mg/mL BSA in 50 mM Tris-HCl, pH 8.5 at 2 min residence time 3 5 mg/mL human IgG in 50 mM sodium acetate, pH 4.5 at 2 min residence time
Separation of Model Proteins (Ovalbumin, ß-lactoglobulin, Cytochrome C and Lysozyme) on 1 mL PRC S HyperCel Prepacked Columns and Scale Up on 5 mL Column, Constant Residence Time of 2 Minutes
Columns: Pall S HyperCel PRC prepacked columns of 1 mL (5 mm I.D. x 50 mm) and 5 mL (8 mm I.D. x 100 mm). Load: for 1 mL column: 100 µL ovalbumin (10 mg/mL), ß-lactoglobulin (10 mg/mL), cytochrome C (2.5 mg/mL) and lysozyme (5 mg/mL). For 5 mL column: 500 µL of same proteins. Equilibration: 50 mM Na acetate, pH 4.5. Elution: 50 mM Na acetate, pH 4.5 + 0.5 M NaCl by linear gradient from 0 to 100%.
Pall offers a comprehensive line of chromatography columns from lab to process scale.
PRC Columns 1 - 5 mL (prepacked) Fast selectivity screening