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Micro-24 MicroReactor System
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The Micro-24 MicroReactor system runs up to 24 simultaneous bioreactor experiments with independent control of each reactor's gas supply, temperature and pH. It is designed for easy set-up and operation using single-use reactor cassettes, continuously monitoring, logging, and controlling each reactor's dissolved oxygen, temperature and pH.
Proprietary Micro-24 MicroReactor software allows the Micro-24 system to be programmed and monitored using a standard laptop or PC computer.
Features
- Single-use MicroReactor Cassettes:
- 24 reactors per cassette
- pH and DO sensors pre-calibrated
- Each cassette is pre-sterilized
- Working volume is 3 to 7 mL
- Independent control of each MicroReactor:
- Dissolved oxygen
- pH
- Temperature
- System-level control of agitation
- Real-time monitoring and control
The Micro-24 MicroReactor Instrument
The Micro-24 MicroReactor comprises of an orbiting base plate with:
- Vacuum seals (15)
- 1 gas injection port per well
- 1 thermal heater per well
- 1 temperature sensor per well
- 1 pH optical sensor per well
- 1 DO optical sensor per well
For More Information
For more information on the Micro-24 MicroReactor, or to arrange a call with a Pall Process Development Specialist, please contact us at bioreactors@pall.com
Product Applications
Pall Micro-24 MicroReactor systems have been used to grow a wide range of cell types under highly diverse conditions. Our systems offer significant advantages over shake flask screening for fast growing organisms and high output processes (e.g. E coli, Bacillus subtilis, cell-free expression systems). The Micro-24 MicroReactor system can also be used for longer term mammalian and insect cell culture, providing high throughput capabilities for controlled process development.
In addition to the highly aerobic processes common to industrial fermentation, Pall MicroReactor systems are also being used to study environmentally interesting organisms (e.g. Shewanella oneidensis MR-1) under controlled anaerobic and micro-aerobic conditions.
Clone Selection
For most companies, clone production easily outpaces the ability to evaluate the potential of the clones. The Pall Micro-24 MicroReactor technology offers the ability to screen hundreds to thousands of clones for growth and productivity in controlled and monitored reactors. Thorough evaluation in a controlled reactor dramatically enhances the chances of selecting the best clones and minimizes the risks of unexpected performance during scale-up.
Media development and optimization
Rapidly screen a wide range of media and components to select the appropriate media, determine critical media components, and optimize media formulations.
Parametric optimization
From characterization of growth and specific productivity across a range of conditions to studying the timing of induction or temperature shifts on final productivity, Pall Micro-24 MicroReactors can provide critical process insights faster and earlier in process development.
Microproduction
There are thousands of uses for reproducible production of milligram quantities of target proteins. Common examples include characterization, crystallography, proteomic studies, or the generation of affinity tags. If you need a large number of small protein samples, Pall Micro-24 MicroReactor systems are a very attractive option.
Typical Application Organisms
- Escherichia coli
- Pseudomonas fluorescens
- Saccharomyces cerevisiae
- Pichia pastoris
- Bacillus subtilis
- Shewanella oneidensis MR-1
- Aspergillus nidulans
- Trichoderma reesei
- Aspergillus oryzae
- Cell-free expression systems
- CHO (chinese hamster ovary) and other mammalian cell lines
- Insect cell lines
For More Information
For more information on the Micro-24 MicroReactor, or to arrange a call with a Pall Process Development Specialist, please contact us at bioreactors@pall.com
Micro-24™ MicroReactor System Specification
| Dissolved Oxygen | |
| Air/oxygen feed | 0 - 20 sccm |
| DO setpoint range | 0 - 100% air saturation |
| DO accuracy | ± 5% at 0% air saturation, ± 10% at 100% air saturation |
| pH | |
| pH setpoint range | 6.0 - 8.0 |
| pH sensitivity | ± 0.1 pH unit |
| pH control accuracy (standard conditions) | ± 0.2 pH unit (at pH 7.0) |
| Temperature (per Well) | |
| T setpoint range | 18 °C - 45 °C |
| T sensitivity | ± 0.1 °C |
| T differential between adjacent wells | Max 2 °C |
| System | |
| Shaker | 0-800 rpm programmable; 5mm orbital throw |
| Power requirements | 110V , 5 A service, or 220 V, 2 A service |
| Air requirements | Clean Dry Air <1.5 CFM, 85-110 psi, Control Gases 0-20 sccm, 15-30 psi 1/4” or 6mm fittings |
| Size | 16 in, wide, 11 in. tall, 17 in. deep |
| System Weight | 22.5 Kilograms |
For More Information
For more information on the Micro-24 MicroReactor, or to arrange a call with a Pall Process Development Specialist, please contact us at bioreactors@pall.com
24-Well Cassette Options and Details
Three types of cassette:
- Non-baffled (microbial)
- Baffled (microbial)
- Central vent (mammalian and insect)
Polycarbonate, irradiated by e-beam
Bottom sectional of cassette showing:
- 1 sealed hydrophobic 0.2 µm membrane per well
- 2 thermal conductors per well
- 1 pH sensor per well
- 1 DO sensor per well
Pall Micro-24 MicroReactor Microbial Cassette: Individual Reactor Detail
Schematic diagram illustrating the functioning of the Micro-24 MicroReactor microbial cassettes. Each of the gas feeds is controlled individually (3 controls per reactor). For microbial applications, pH is controlled by injecting either dilute ammonia or carbon dioxide directly into the culture medium through the membrane at the bottom of each reactor well. Oxygen control is via the same injection site.
Pall Micro-24 MicroReactor Cell Culture Cassette: Individual Reactor Detail
Schematic diagram illustrating the functioning of the Micro-24 MicroReactor cell culture cassette. Up to 3 gas feeds can be individually controlled per reactor. The gases (or gas mixtures, depending on application) are injected through the sterilizing membrane, flow through the vent tube into the headspace, and diffuse into the culture medium during orbital agitation.
Four Options for Well Closures
Type A: Single-use with plastic check valve and sterile membrane barrier
Type D: Single-use with sterile membrane barrier
Type E: Autoclavable, re-usable, with stainless steel check valve, no sterile membrane barrier
Abgene Tape: Single-use, good for high gas exchange conditions, short experiment durations
Control Software
Individual set points for temp, pH, DO for each of the 24 reactors
Real-time monitoring of temp, pH, DO and gas fluxes
For More Information
For more information on the Micro-24 MicroReactor, or to arrange a call with a Pall Process Development Specialist, please contact us at bioreactors@pall.com
Pall Micro-24 MicroReactor Part Numbers
Systems
| Part Number | Description |
| MRT-MICROSYS-A | Complete Microbial System - US |
| MRT-CCSYS-A | Complete Cell Culture System-US |
| MRT-MICROSYS-M | Complete Microbial System - Metric |
| MRT-CCSYS-M | Complete Cell Culture System-Metric |
Hardware
| Part Number | Description |
| MRT-M24-A | Micro-24 MicroReactor, non-metric (US) |
| MRT-M24-M | Micro-24 MicroReactor, metric (EU/Asia) |
| MRT-CMP | Laptop Controller w/Micro-24 MicroReactor software |
| MRT-APV-3 | Ammonia Pressure Vessel, non-metric (US) |
| MRT-APV-3M | Ammonia Pressure Vessel, metric (EU/Asia) |
| MRT-BOX-1 | Reusable ABS Shipping Container with custom foam |
| MRT-KIT-A | Installation Kit, non-metric (US) |
| MRT-KIT-M | Installation Kit, metric (EU/Asia) |
Consumables
| Part Number | Description |
| MRT-REG-2CS | Case of 6 Standard Cassettes |
| MRT-BFL-2CS | Case of 6 Baffled Cassettes |
| MRT-PRC-2CS | Case of 6 Cell Culture Cassettes |
| MRT-TPE-1 | Set of 6 sterile sealing tapes |
| MRT-CAP-A6 | Case of 6x24 Type A Caps |
| MRT-CAP-D6 | Case of 6x24 Type D Caps |
| MRT-CAP-E24 | Set of 24 Type E Caps - autoclavable |
Services
| Part Number | Description |
| PTS02MRTSVCINST | Micro-24 Installation Service |
| PTS02MRTSVCTRN | Micro-24 Start-up Training, per day |
| PTS02MRTSVCRNT | Micro-24 Rental, 4 weeks |
For More Information
For more information on the Micro-24 MicroReactor, or to arrange a call with a Pall Process Development Specialist, please contact us at bioreactors@pall.com