Single-pass tangential flow filtration (TFF) is revolutionizing how TFF is implemented in biotech, vaccine, blood plasma and other applications. Pall is now making it even easier to implement this breakthrough patented technology into your process. As with the original Cadence single-pass TFF module, the new Cadence Inline Concentrator allows direct flow-through in-process volume reduction and can be implemented into a process system or operate as a stand alone unit.
This exciting new technology leads to economic and practical benefits, including:
Ability to couple the concentration of product before or after other Downstream Processing (DSP) steps, consequently optimizing other steps and reducing in-process pool tank volumes.
Continuous processing in DSP
Easy integration of the assembled module, allowing plug and play
Potential reduction of product damage or aggregation due to reduced residence time and shear exposure.
The preassembled Inline Concentrator modules do not need a holder. Once the feed port is connected to a pressurized feed source, the module is ready for use. The built-in resistor in the retentate manifold eliminates the need for downstream instrumentation and allows volume reduction of 2 to 4X (or higher), depending on the application. Pall can provide tubing sets to ease the testing and implementation of the Cadence Inline Concentrator.
Using Cadence Inline Concentrator with Delta membrane, the innovative benefits of single-pass TFF technology are enhanced by the proven high flux, high selectivity, and low protein binding attributes of Delta regenerated cellulose membrane.
Optimize Economic and Operational Benefits
Enhance downstream processing to increase capacity and reduce costs – Cadence Inline Concentrator modules can help to eliminate or reduce the size of intermediate storage tanks and associated cleaning of tanks when used for in-process volume reduction before or after existing steps.
Enable in-process volume reduction – Depending on the initial concentration and product characteristics, a volume reduction of 2 to 4X (or higher) can be achieved with minimal instrumentation or system.
Optimize processing of highly shear-sensitive products – Processing results in only one pass through the pump and module, reducing shear exposure. For products sensitive to pumping, the pump can be completely eliminated by using pressurized vessels to flow the process fluid through the module. Further benefits are achieved by eliminating any mixing or foaming issues associated with the feed tank.
No holder required – Cadence Inline Concentrator modules are provided assembled, and the cassettes and manifolds are pre-torqued between two end plates so that no extra holder is required.
Easy connection to a pressure source, like a pump or pressure vessel
Simplified in-process volume reduction
Continuous processing enabled by process coupling
The module feed, retentate and permeate ports are easily connected to the appropriate outlets using clearly marked ports
Innovative single-pass TFF processing takes place within the Cadence SPTFF modules. Cadence SPTFF modules incorporate Pall’s proven T-Series cassettes with Delta membrane, and are available in different formats to accommodate various processing volumes (see Ordering Information).
T-Series Cassettes with Delta Membrane
Delta regenerated cellulose membrane offers high flux and selectivity. The membrane has been specifically developed to minimize protein binding to the surface and interstitial structure of the membrane. Inherently hydrophilic, this membrane shows low protein adsorption properties and is optimal for processes involving very hydrophilic proteins.
The Cadence Inline Concentrator is designed for continuous processing or for in-process volume redution in various steps in a wide range of applications in the biopharmaceutical industry including recovery of antibodies or recombinant proteins and preparation of samples (concentrating, desalting) before or after column chromatography and can be implemented in existing facilities facing tank capacity issues or to optimize existing processes.
The operating conditions for any single-pass TFF process must be established by performing trials and analyzing results. Pall’s Technical Service Group is available to assist in conducting trials to develop operating conditions necessary to achieve the desired process objectives.
Materials of Construction
Cassette: Delta regenerated cellulose membrane, 10 kD or 30 kD with polypropylene screen, white polyurethane encapsulant with white pigment (TiO2), and medical grade silicone for the permeate seals
Gaskets: Medical grade, platinum cured silicone
Manifold Plates: UHMW polyethylene
Tubing and Connectors:
T01/T02 - female luer connection and cap: Polypropylene
T12 - MPC quick disconnect connector and plug: Polysulfone
T06 - MPX quick disconnect connector and plug: Polysulfone
Maximum Pressure: 4.1 barg (60 psig)
Maximum Transmembrane Pressure (TMP): 4.1 barg (60 psig)
Processing Temperature Range: 4 – 40 °C (freezing will damage module)
pH Range: 2 – 13
Typical Feed Flow Rates
JFEED, DIW [LMH] (20-60 psig)
0.1 - 0.3
0.2 - 0.7
0.9 - 3.5
4.7 - 17.5
Module Integrity Test
Module Integrity Test Pressure: 4.1 barg (60 psig)
Each Cadence module has a unique serial number for full traceability.
The shelf life of Cadence Inline Concentrator packaged in preservative is expected to be 1 year from the date of manufacture when the modules are stored unopened in the original packaging at temperature up to 25 °C and protected from direct light. Extended shelf life studies are ongoing.
Materials of construction for Cadence Inline Concentrator modules have been tested and meet the requirements for the Biological Reactivity Tests listed in the United States Pharmacopeia (USP) under USP <88> for Class VI - 70°C Plastics.
Each module is supplied with the following comprehensive documentation to ensure the Cadence Inline Concentrator module is operated successfully:
Certificate of Quality
Care and Use Procedures
Material Safety Data Sheet for the Module Preservative Solution
Contact your local Pall representative to obtain:
Validation Service for specific tests such as compatibility testing with your product fluid
Training and Technical Support to optimize your process using Cadence Inline Concentrators
Cadence Inline Concentrator Concentration Factor vs. Permeate Flux at Polyclonal Bovine IgG Feed Concentrations of 2 and 10 g/L over Various Feed Pressures (20 – 60 psig)
Case Study: 2 g/L Polyclonal Bovine IgG Processing with Delta 30 kD, T01 (0.065 sqm.) Module
Case Study: 5 g/L Polyclonal Bovine IgG Processing with Delta 10 kD, T06 (3.5 sqm.) Module
Identify and order Cadence Inline Concentrator using the table below.
Guide to Cadence Inline Concentrator Module Part Numbers IL Inline concentrator D Delta regenerated cellulose membrane 010 Nominal molecular weight cut-off T01 Cassette format 04 Number in series 07 Number of cassettes
For example, a T01 module with 10 kD Delta regenerated cellulose membrane area of 0.065 m2 (0.7 ft2) is part number ILD010T010407.
Cadence Inline Concentrator Part Number and Membrane Area (m2)