Tangential Flow Filtration Introduction
The fluid dynamics of cross flow filtration reduces membrane fouling and maintains filtration rates (flux) for a longer period of use, thus increasing membrane throughput (greater capacity) compared to traditional cartridge (direct flow) filtration. Additional advantages of TFF include the ability to reuse the filter modules and relatively low capital costs.
TFF can be applied to a wide range of biological fields. It is applicable to R&D discovery applications right through pilot and full-scale production. TFF can be used to concentrate and desalt sample solutions ranging in volume from a few milliliters to thousands of liters. It can be used to fractionate large from small biomolecules, harvest cell suspensions and clarify fermentation broths and cell lysate.
Microfiltration and ultrafiltration processes incorporating tangential flow or cross flow filtration is utilized in a wide range of biopharmaceutical applications. Examples of a few typical applications are listed below:
- Concentration and desalting of protein, peptide, and oligonucleotide solutions
- Purification and recovery of antibodies or recombinant proteins
- Vaccine and conjugate concentration and diafiltration.
- Fractionation of protein mixtures
- Blood plasma fractionation and purification
- Cell broth clarification, concentration
- Cell culture perfusion such as in monoclonal antibody (Mab) production
- Clarification of Fermentation broths
- Concentration and washing of bacterial cells
- Water and buffer purification (endotoxin removal)
Ultrafiltration ApplicationsOmega™ polyethersulfone (PES) and Delta regenerated cellulose membrane cassettes and their associated hardware and systems are the first choice in applications that require precise tailoring of membrane characteristics and gentle processing. Membrane cassettes are the preferred technology for concentration and diafiltration applications for recombinant proteins and other biological process fluids.
Microza hollow fibers offer advantages for endotoxin removal from buffer preparation and pharmaceutical water applications.
Microfiltration ApplicationsPallSep™ Biotech systems provide gentle, efficient separation in complex biotech harvest and clarification applications such as the concentration, clarification, and purification of high particle load, high viscosity or difficult-to-filter solutions such as fermentation broths or lysates.
Microza* hollow fiber modules are the preferred choice for clarification of fermentation broths and cell culture fluids. They are often used for concentration, washing and recovery of bacterial cells prior to lysing and recovery of inclusion bodies and recombinant proteins from the cells. Reliability, mechanical strength, simple flow geometry and steam sterilizability make Microza hollow fiber modules an excellent option for perfusion cell culture.
Supor® TFF suspended screen channel membrane cassettes can be used in batch applications for the harvesting of bacterial, mammalian or insect cells. They may also be used for lysate clarification.
*Microza is a trademark of Asahi Chemical Company.