Affinity Chromatography

Affinity chromatography separates compounds based on the properties of chosen ligands with specific affinity to the molecule being selected for attachment to the solid matrices. Often, the binding between the molecule and the ligand can be reversed after the selection step. This is done, for example, through buffer, salt concentration, or pH change. Pall offers bottled resins and packaged kits for affinity chromatography. Pall bottled resins can be used for small and large sample sizes, and for single use and high throughput methods of purification. Our base resin varies depending on targeted application.

 
Type of Affinity Purification Ligand Pall Product
Albumin Depletion Blue Dye
IgG Purification Protein G
Lipoprotein Purification Heparin
Glycoprotein Purification Lysine
 

Affinity Chromatography Products

Blue Trisacryl® M Affinity Chromatography Resin
Blue Trisacryl® M Affinity Chromatography ResinBlue Trisacryl® M Resin is an affinity chromatography resin used for the purification of a wide variety of enzymes and proteins such as kinases, albumin, interferons, and some coagulation factors. The basic matrix is Trisacryl GF2000, a macroporous non-ionic resin on which Cibacrons Blue is covalently immobilized. Cibacron Blue F3GA dye is strongly bound to the matrix through a six carbon spacer arm. The reaction is performed with the EEDQ coupling agent. This type of coupling prevents any leakage of the dye in normal working conditions.




Heparin HyperD M Affinity Chromatography Resin
Heparin HyperD M Affinity Chromatography ResinHeparin HyperD M Resin is a high speed, high capacity affinity preparative resin for the purification of biological molecules that bind to heparin, such as coagulation factors, growth factors, lipoproteins, etc. The resin provides high binding capacity at high flow rates. Heparin HyperD M affinity resin employs a high-capacity hydrogel polymerized within the large pores of a rigid bead. This design combines the desirable characteristics of a soft, high capacity hydrogel with the high dimensional stability of a rigid bead.





Lysine HyperD Affinity Chromatography Resin
Lysine HyperD Affinity Chromatography ResinLysine HyperD Resin is a high speed, high capacity affinity preparative resin for the purification of biological molecules that bind to lysine such as plasminogen from human or animal species plasma. The resin provides high binding capacity at high flow rates. Lysine HyperD affinity resin employs a high-capacity hydrogel polymerized within the large pores of a rigid bead. This design combines the desirable characteristics of a soft, high capacity hydrogel with the high dimensional stability of a rigid bead.





Chromatography
Chromatography
An overview of Pall's chromatography technologies for lab-scale applications.
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