Bottled (Bulk) Chromatography Resins
Simplify protein purification and fractionation for biomarker discovery, proteomics research, and drug development
Designed for affinity, ion exchange, size exclusion, hydrophobic interaction, and hydroxyapatite chromatography applications, these products offer:
The chromatography resins below are available in packages from 25 mL to 1000 mL for laboratory applications and can be scaled up to the largest production scales to accommodate the volumes and processes found in the biopharmaceutical industry.
Affinity Chromatography ResinsBlue Trisacryl® M Chromatography Resin
An affinity chromatography resin used for the purification of a wide variety of enzymes and proteins such as kinases, albumin, interferons, and some coagulation factors. The basic matrix is Trisacryl GF2000, a macroporous non-ionic resin on which Cibacrons Blue is covalently immobilized. Cibacron Blue F3GA dye is stronglybound to the matrix through a six carbon spacer arm. The reaction is performed with the EEDQ coupling agent. This type of coupling prevents any leakage of the dye in normal working conditions.
A high speed, high capacity affinity preparative resin for the purification of biological molecules that bind to heparin, such as coagulation factors, growth factors, lipoproteins, etc. The resin provides high binding capacity at high flow rates. Heparin HyperD M affinity resin employs a high-capacity hydrogel polymerized within the large pores of a rigid bead. This design combines the desirable characteristics of a soft, high capacity hydrogel with the high dimensional stability of a rigid bead.
A high speed, high capacity affinity preparative resin for the purification of biological molecules that bind to lysine such as plasminogen from human or animal species plasma. The resin provides high binding capacity at high flow rates. Lysine HyperD affinity resin employs a high-capacity hydrogel polymerized within the large pores of a rigid bead. This design combines the desirable characteristics of a soft, high capacity hydrogel with the high dimensional stability of a rigid bead.
Protein A Ceramic HyperD® F
A high capacity affinity resin designed for process-scale purification of immunoglobulin G. The resin combines ease of use with high binding capacity and excellent scalability. Protein A Ceramic HyperD F resin is prepared using a rigid proprietary ceramic bead. Recombinant Protein A is immobilized to a specially formulated hydrogel within the porous ceramic bead.
Ion Exchange ChromatographyCeramic HyperD®
Gel-in-a-shell design delivers outstanding dynamic capacity and exceptional dimensional stability for unsurpassed productivity. Proteins diffuse rapidly within the hydrogel, facilitating rapid uptake of product, a process known as enhanced diffusion.
Mixed-mode ChromatographyHA Ultrogel®
A hydroxyapatite agarose composite resin for the separation of biomolecules from research and development scale to manufacturing. HA Ultrogel is a cross-linked tridimensional composite based on spherical agarose beads with entrapped microcrystals of hydroxyapatite.
HEA and PPA HyperCel™
Novel industry scalable chromatography resins designed for protein capture and impurity removal in a biopharmaceutical environment. Operating on a "mixed-mode" mechanism, their chromatographic behavior is based on a combination of electrostatic and hydrophobic properties of the protein and ligands. HEA and PPA HyperCel resins provide unique and different selectivities not accessible with traditional ion exchange or HIC, that can be screened to facilitate process development. For example, the mixed-mode interaction mechanism can be exploited to achieve discrimination of proteins having similar or very close isoelectric points, a separation which cannot be performed by methods like an ion exchange.
A high capacity, high selectivity resin designed for the capture and purification of monoclonal and polyclonal antibodies. HCIC is based on the pH dependent behavior of ionizable, dual-mode ligands. MEP HyperCel resin carries an antibody-selective ligand, 4-Mercapto-Ethyl-Pyridine (4-MEP). Adsorption is based on mild hydrophobic interaction and is achieved without addition of lyotropic or other salts. Desorption is based on charge repulsion. It is performed by reducing the pH.
Unique resin designed to eliminate solvent and detergent from biological fluids. SDR HyperD resin is made of silica-beads in which the pore volume is filled with a three-dimensional cross-linked hydrophobic polymer. The particle size distribution (40-100 µm), the small pore size of the silica beads, and the hydrophobic nature of the chemical groups make SDR HyperD resin an excellent tool for specific solvent detergent removal from biological liquids.