Centrifugal Devices Facilitate Pure, Concentrated Product with High Recoveries
Pall’s ultrafiltration centrifugal devices simplify many common protein handling procedures. These devices provide efficient concentration and salt removal of samples from 50 μL to 60 mL in just minutes. Choose from membranes that have been developed to assure low nonspecific biomolecule binding and provide consistent, high recovery of target molecules. Ultrafiltration reduces the amount of handling that can cause damage to samples, leaving concentrated samples ready for direct incorporation into downstream applications at critical stages in the discovery process.
Pall’s microfiltration centrifugal devices are used in protein separation and small -scale general filtration procedures. These devices can be used in combination with chromatography resins to create a fast, efficient method for purifying proteins of interest.
Rapid ProcessingAchieve high recoveries in as little as five to ten minutes.
High Performance MembranesOmega™ polyethersulfone ultrafiltration membrane provides higher flow rates and is lower protein binding than competitive membranes. This results in lower processing time and the highest possible recoveries.
Low Protein BindingDevices are constructed of low-binding materials to maximize sample recovery.
Variety of MWCO’s and Pore SizesAvailable with ultrafiltration membranes for rapid concentrating and/or desalting of proteins. Also available with low-binding microfiltration membranes for particulate removal or chromatography separations.
Easy to UseOnce you have identified the right MWCO or pore size ranging from 1 kD to 0.45 μm, the devices are color-coded for easy visual identification.
Ideal for Fast Batch Mode Chromatography ApplicationsNanosep® centrifugal devices with microfiltration membrane serve as a perfect housing for chromatography resin. The spin device can be filled with the resin of choice to perform the desired protein purification application.
Nanosep Devices Exhibit Fast Spin Times and High Recoveries
|Solute||Solute MW (kd)||Spin Time (min.)||15||10||8||5||3|
|Vitamin B12||1,335||% Recovery||7||-||-||-||-|
|Cytochrome C||12,400||% Recovery||100||89||77||1.8||-|
|Chymotrypsinogen A||25,000||% Recovery||-||97||94||2.1||-|
|Thyroglobulin (1 mg/mL)||677,000||% Recovery||-||-||-||100||91|
Samples of 0.5 mL of a 1.0 mg/mL solution were centrifuged at 14,000 x g and concentrated to a volume of 10 to 60 μL using Nanosep centrifugal devices with Omega membrane.
Match Device Size to Sample VolumePall’s centrifugal devices are available in a range of sizes to accommodate your specific sample volumes. AcroPrep™ filter plates can be used with smaller sample volumes in similar applications.
|AcroPrep 384 filter plate||< 100 µL|
|AcroPrep 384 filter plate||< 350 µL|
|AcroPrep 384 filter plate||< 1 mL|
|Nansep device||< 0.5 mL|
|Microsep™ device||0.5 - 3.5 mL|
|Macrosep ® device||3 - 15 mL|
|Jumbosep ™ device||15 - 60 mL|
Easy-to-assemble Stirred Cell Systems Process 2 to 150 mLPall’s Stirred Cell Systems provide a versatile format that can be disposed of when working with biologically hazardous or radioactive materials, or cleaned and reused up to 20 times. These devices are 100% integrity tested to ensure the membrane and cell reservoir are integral. Ultrasonically sealed membranes eliminate the need for O-rings that can leak. The devices are easy to assemble, use and clean up, and feature as much as 50% more effective filtration area than conventional stirred cells of the same volume.
- Concentrate, purify and desalt peptides and proteins
- Separate proteins from acrylamide gels
- Prepare samples for HPLC analysis
- Fractionate proteins