Drinking Water Quality Control: Total Coliforms and Fecal Coliforms/E. coli Detection

Total Coliforms (Method Number 9222)

MF-Endo Broth: E. coli, 24 hour culture at 35 °C MF-Endo Broth: E. coli, 24 hour culture at 35 °C 










Recommended Media Broth:

Broth MF-Endo
Target Organism Total Coliforms
Recovery (versus control) > 85%
Test Organism E. coli
pH at 25 °C 7.2 +/- 0.2
Shelf Life (2 - 8 °C) 1 year
Media Color Pinkish red
Target Colony Color Dark red with metallic sheen

Fecal Coliforms/E. coli (Method Number 9222)

M-FC Broth: E. coli, 24 hour culture at 44.5 °C
M-FC Broth: E. coli, 24 hour culture at 44.5 °C 
 















Pall Preferred Online Lab Community

M-FC Broth with rosolic acid: E. coli, 24 hour
M-FC Broth with rosolic acid: E. coli, 24 hour culture at 44.5 °C











Recommended Media Broth:

Broth MF-C/ MF-C with rosolic acid
Target Organism Fecal Coliforms
Recovery (versus control) > 85%
Test Organism E. coli
pH at 25 °C 7.4 +/- 0.2
Shelf Life (2 - 8 °C) 1 year
Media Color Blue/violet
Target Colony Color Blue

Recommended Filters:

GN-6 Metricel® Membranes
GN-6 Metricel Membranes Mixed cellulose ester GN-6 Metricel® membranes set the standards worldwide for meeting stringent regulations by providing uniform and consistent growth of organisms. 



MicroFunnel™ Filter Funnels
MicroFunnel Filter FunnelsMicroFunnel easy-to-use, disposable filter funnels.




Magnetic Filter Funnels
Magnetic Filter FunnelsPall’s unique Magnetic Filter Funnel is used extensively throughout the water quality testing community because of its one-handed operation that simplifies testing and improves aseptic technique. .