HCIC is based on the pH-dependent behavior of ionizable, dual-mode ligands. Adsorption is based on mild hydrophobic interaction and is achieved without addition of lyotropic or other salts. Desorption is based on charge repulsion. It is performed by reducing pH.
MEP HyperCel™ Resin
MEP HyperCel™ Resin is a high capacity, high selectivity resin designed for the capture and purification of monoclonal and polyclonal antibodies. HCIC is based on the pH dependent behavior of ionizable, dual-mode ligands. MEP HyperCel resin carries an antibody-selective ligand, 4-Mercapto-Ethyl-Pyridine (4-MEP). Adsorption is based on mild hydrophobicinteraction and is achieved without addition of lyotropic or other salts. Desorption is based on charge repulsion. It is performed by reducing the pH.
HEA and PPA HyperCel Resins
HEA and PPA HyperCel Resins are novel industry scalable chromatography resins designed for protein capture and impurity removal in a biopharmaceutical environment. Operating on a "mixed-mode" mechanism, their chromatographic behavior is based on a combination of electrostatic and hydrophobic properties of the protein and ligands. HEA and PPA HyperCel resins provide unique and different selectivities not accessible with traditional ion exchange or HIC, that can be screened to facilitate process development. For example, the mixed-mode interaction mechanism can be exploited to achieve discrimination of proteins having similar or very close isoelectric points, a separation which cannot be performed by methods like an ion exchange.