Multi-Well Membrane-Bottom Filter Plates

As samples get smaller and more numerous, the need for novel methods to purify samples and improve assays has led Pall to develop a broad line of multi-well filter plate platforms targeting specific application challenges. 

The AcroPrep™ and AcroWell™ families of filter plates feature individually-sealed membranes that eliminate crosstalk and solution weeping. The proprietary sealing technology allows Pall to seal virtually any type of membrane or media configuration into these device platforms to meet ever-changing industry needs.

 







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Features for Optimizing Filter Plate Applications

 
  • Broad selection to suit your application: Available in 96- or 384-well formats with a variety of single- and multi-layer membranes, plate color choices, well volumes, and outlet tip lengths for use in a multitude of sample preparation and detection processes.
  • Automation friendly: Designed in accordance with the standards of the ANSI/SBS X-2004. Rigid construction enables the plates to be easily maneuvered by robotic equipment and assures that the plates do not flex and jam detectors or other instrumentation.
  • Low binding, chemically resistant: Polypropylene housing assembly is compatible with a broad range of aqueous and organic liquids, and is noted for its low biomolecule binding, which minimizes non-specific adsorption to the plate.
  • Serialized barcodes: Labels allow for the use of automated tracking systems.
  • No crosstalk: Specially engineered fluid directors and outlet tips on the bottom of the AcroPrep plate are designed to reduce the potential for crosstalk. Elimination of crosstalk is assured through individually sealing a membrane in each well using a proprietary sealing technology.

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See the AcroPrep™ Filter Plate in Action



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Methodology: Filtration versus Retention

  1. Place plate on vacuum manifold or hold the plate so the outlets on the bottom of the plate are not touched.
  2. Add sample and incubate. Apply vacuum (or centrifuge).
  3A. Release vacuum from the manifold. Remove filter plate and retained sample for further processing. (Or, 3B)
  3B. Release vacuum from the manifold. Remove filter plate. Remove collection (receiver) plate and utilize collected filtrate in downstream applications.

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AcroPrep™ or AcroWell™ Filter Plate... What's the difference?

AcroPrep Filter Plate Design

       
  • Membranes are individually cut, placed, and sealed in the wells.
  • Plates can be used in both filtrate and retentate-based applications.
  • Plates have either long or short fluid directors to direct the flow of the filtrate without cross-contamination.
  • Proprietary sealing process allows flexibility in sealing a wide range of membrane both single and multiple layers.
  • Available in a wide range of configurations to meet various application challenges.
AcroWell Filter Plate Design

       
  • Constructed of two membrane layers - the bottom layer of membrane protects the upstream membrane and acts as a barrier to passive flow.
  • Designed for use in retentate-based applications such as hybridization-based binding assays and time resolved fluorescence (TRF).
  • Plate and membrane contruction allows extended or elevated-temperature incubations without solution weeping and crosstalk.


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Filter Plate Outlet Tip Types

AcroWell 96 AcroPrep 96 AcroPrep 96
1 mL
AcroPrep 384
Long Tip
AcroPrep 384
Short Tip
Use for retentate-based applications. Not recommended for filtrate collection. Use for both filtrate- and retentate-based applications. Outlet tips are recessed beneath the skirt of the plate. Use for both filtrate- and retentate-based assays. Outlet tips extend beyond the skirt of the plate and pilot into the wells of a receiving plate. Use for both filtrate- and retentate-based applications. Outlet tips extend beyond the skirt of the plate and pilot into the wells of a receiving plate. Use for both filtrate- and retentate-based applications. Outlet tips are recessed beneath the skirt of the plate.

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Link to Filter Plate Datasheets and Ordering Information

Click on links below for specifications and ordering information.
 
Filter Plate Description
AcroPrep 96 Filter Plates – 350 µL 96 wells, 350 µL well volume; for filtrate and retentate-based applications
AcroPrep 96 Filter Plates – 1 mL 96 wells, 1 mL well volume; for filtrate and retentate-based applications requiring larger per well volume processing
AcroWell 96 Membrane-bottom Plates 96 wells, 350 µL well volume; for use in retentate-based applications such as hybridization-based binding assays and time resolved fluorescence
AcroPrep 384 Filter Plates 384 wells, 100 µL well volume; for filtrate and retentate-based applications
Multi-well Filter Plate Vacuum Manifold and Accessories Optimized for use with Pall filter plates
 

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Literature and Technical Support


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Typical Applications
  • Sample prep/gross fractionation
  • DNA purification
  • Receptor:ligand binding assays
  • Prefiltration
  • Clarification of cell lysates and tissue homogenates
  • Preparation of proteinaceous solutions
  • Drug binding studies
  • Low protein or nucleic acid binding procedures
  • Combinatorial chemistry library screening
  • Cleavage assays
  • Bead-based assays
  • Size exclusion assays
  • Concentration, purification and desalting of proteins and nucleic acids
  • Molecular synthesis
  • ELISA
  • ELISPOT