Mustang® Membrane Devices for Ion Exchange Chromatography

Fast, reliable performance in scalable device formats

Membranes are recommended in chromatography applications when there is a need to purify large molecules or in situations where faster flow is needed. Membrane chromatography is extremely economical because flow rates are significantly faster than traditional resin chromatography, decreasing cycle time and increasing throughput. Pall’s Mustang membranes possess large convective pores and have dynamic binding capacities that are relatively insensitive to the effects of high flow rates, even for large molecules such as plasmids and viruses. Pall’s membrane devices for ion exchange chromatography offer:

  • True scalability – For laboratory-scale applications, Mustang membranes are available in Acrodisc® units for single samples and AcroPrep™ filter plates for higher sample processing. Devices with Mustang S and Mustang Q membranes can be scaled up to larger-capacity capsules and cartridges from Pall.
  • Application-specific membrane chemistries – Mustang Q membrane is a strong anion exchanger that effectively binds plasmid DNA, negatively-charged proteins, and viral particles. Mustang S membrane is a strong cation exchanger that effectively binds positively-charged proteins and viral particles.
  • High binding capacities and fast flow rates – Mustang membranes withstand high flow rates to render faster purification without affecting recovery rates.

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Mustang S Membrane Products for Cation Exchange

High capacity Mustang S membrane is a cation exchanger with a polyethersulfone (PES) base modified with sulfonic acid groups. Mustang S membrane delivers efficient and rapid flow rates with a convective pore structure combined with high dynamic binding capacity for positively-charged proteins (10 mg) and viruses. Processing time is much shorter and more efficient than the conventional bead or resin-based technology. The syringe filter and multi-well plate formats can directly scale up to larger-scale capsules and cartridges for larger-volume applications. Device options:

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Mustang Q Membrane Products for Anion Exchange

Mustang Q membrane is an anion exchanger with a polyethersulfone (PES) base modified with quaternary amines. Mustang Q membrane delivers efficient and rapid flow rates with a convective pore structure combined with high dynamic binding capacity for plasmid DNA (3.6 mg/Acrodisc unit), negatively-charged proteins (10 mg), and viruses (1012viral particles). Processing time is much shorter and more efficient than the conventional bead- or resin-based technology. Mustang devices have throughputs of up to 100 times that of traditional columns, with no associated loss of binding capacity. The syringe filter and multi-well plate formats can directly scale up to larger-scale capsules and cartridges for larger-volume applications. Device options:

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Mustang membrane products can be used for applications including:

  • Contaminant removal such as DNA viral particle, host cell proteins, or endotoxin.
  • Isolation via capture and release of plasmid DNA, virus or target protein from a complex mixture.
  • Protein fractionation or capture.
  • Antibody purification.