Sensitive Protein Detection with Low Background and Very Low Burnthrough
Naturally hydrophobic polyvinylidene fluoride is ideal for a wide variety of protein-analysis applications. The family of FluoroTrans media are white, microporous solid phase supports that bind proteins tenaciously via hydrophobic interactions.
High sensitivity for small peptides.
High protein binding capacity. Typically absorbs 50% more protein than nylon or nitrocellulose.
FluoroTrans PVDF membrane is optimized for N-terminal protein sequencing. The medium demonstrates good signal-to-noise ratios with standard detection systems, and immobilized proteins can be used directly for sequencing, or visually detected with common staining reagents including Amido Black, Ponceau S, and colloidal gold.
FluoroTrans W membrane is optimized for Western transfer applications. This membrane allows for sensitive protein detection with low background and very low protein burnthrough. Immobilized proteins can be visually detected with Coomassie♦ blue, Amido Black, Ponceau S, and colloidal gold.
FluoroTrans Membrane Has Excellent Sensitivity, Signal, and Background in Western Transfers
Rabbit reticulocyte lysate (Amersham) was loaded in lanes of polyacrylamide gels at f.s., 1/3 and 1/10 dilutions. After electrophoresis, proteins were transferred to membranes. Membranes were stained with 0.1% Amido Black, 45% methanol, and 2% acetic acid for 4 minutes; then destained for 5 minutes with two changes of 90% methanol and 2% acetic acid. Stained membranes were rinsed in water and air dried.