Total Bacteria - Microbiological QC Applications

Total Bacteria (Method Number 9215; USP 61, 71)

M-TGE Broth: S. epidermidis, 24 hour culture at 35 °C




Tryptic Soy Broth USP: S. epidermidis, 24 hour culture at 35 ° C



HPC Broth: S. epidermidis, 24 hour culture at 35 °C




Recommended Media Broth:

Broth M-TGE Trypticase Soy USP HPC with HPC indicator
Target Organisms Total Bacteria Total Bacteria Total Bacteria
Recovery (versus control) > 85% > 85% > 85%
Positive Control Test Organism(s) E. coli; S. epidermidis E. coli; E. coli; S. epidermidis
pH at 25 °C 7.0 + 0.2 7.0 + 0.2 7.3 + 0.2
Shelf Life (2 - 8 °C) 1 year 1 year 1 year
Media Color Pale yellow Pale yellow Pale yellow
Colony Color Organism dependent Red Organism dependent

 














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