Polyethersulfone (PES) membrane for aqueous solutions provides removal of fine particles, bacteria, viruses, and fungi making it a versatile membrane for applications such as sample preparation, sterile filtration and infusion therapy. PES is an inherently hydrophilic membrane that wets out quickly and completely resulting in fast filtration with superior flow rates and high throughputs. The hydrophilic nature of PES means no added surfactants are used to increase wettability. PES membrane is also extremely low protein binding minimizing the likelihood of target analyte binding. Polyethersulfone membrane is compatible with EtO, gamma irradiation, and autoclave methods of sterilization.
The low protein binding nature of the polyethersulfone membrane makes it well suited for biological sample preparation. Available in a variety of pore sizes, the membrane can be used for coarse particulate removal in prefiltration applications or as a fine final filter for clarification. An excellent choice for bead-based, multi-plexed assays, the PES membrane yields high microsphere recovery and reduces the incidence of false positives in serological assays.
Available in 0.1 and 0.2 µm pore sizes, the polyethersulfone membrane provides sterilization of buffers, culture media, additives, and pharmaceutical filtration. If mycoplasma contamination is a concern, the 0.1 µm PES membrane provides assurance that critical samples will not be contaminated.
For infusion therapy, the inherent hydrophilicity of the membrane allows fast priming of a finished device and provides a barrier to air passing through the wetted membrane. While wetting out quickly, PES membrane provides high throughputs over time extending the life of the finished product. The membrane has a uniform pore structure for application requirements of sterile fluids and particulate retention. Our polyethersulfone membrane also complies with United States Pharmacopeia (USP) Biological Reactivity Test, In Vivo <88> for biosafety, cytotoxicity, and hemolysis testing.
Available in a modified black format, the PES membrane provides a contrasting membrane for microbiological and particulate analysis. The dark background provides excellent contrast for counting opaque colonies in labs and monitoring light-colored particulate in process fluids. Black PES membrane is an excellent medium for the isolation and enumeration of yeast or mold colonies.
Products in this datasheet may be covered by one or more patents including EP 1,056,540; US 6,878,343; US 7,135,117; and US 7,371,325.
Pall polyethersulfone membrane flow rate outperforms nylon and PVDF membranes of the same pore size (0.2 µm).
Pall PES Membrane is Low in Biomolecule Binding
125I-labeled BSA (1.6 µg) or 32P-labeled DNA (500 ng) was diluted to 5 mL in PBS (BSA) or Tris-EDTA (DNA) and filtered through a 13 mm disc of the indicated membrane. Filtration was carried out using a 10 mL syringe at a flow rate of 1.0 mL/minute. Binding was determined by comparing the amount of radioactivity remaining in the membrane (triplicate) to the activity of the starting material by counting the disc or solution in a scintillation counter. Positively-charged nylon membrane is designed for biomolecule binding and was used as a positive control.
AcroPrep™ 96 Filter Plates with 1.2 µm PES Reduce the Occurrence of False Positives in Multi-Plex Assays
The serological immunoassays were performed with multiple lots of xMAP microspheres in both the AcroPrep 96 with 1.2 µm PES membrane and Competitor M filter plates. The results from these filter plates were read with one Luminex LX100 Instrument. The responses represent the reactivity toward microspheres without proteins coupled to them to maximize the indications of false positive “non-specific” reactivity by the microspheres. The results represent the average of four replicates for each of the three “positive” and one “negative” samples tested. In all lots of microspheres tested, the PES-based filter plates exhibited a marked reduction in non-specific reactivity than competitive plates.
The AcroPrep name is a trademark of Pall Corporation and is not available for use.
Custom roll, sheet, and disc sizes available upon request. Please contact your local sales representative for additional information.