The AcroPrep 24-well filter plate can be used for vacuum processing. The Pall multi-well plate vacuum manifold (part number 5017) is well suited for this application. The recommended operating vacuum is ≥ 25.4 cmHg (10 inHg). In experiments with cultured mammalian cells (CHO and HEK293T), a vacuum of 38.1 cmHg (15 inHg) was found to work well.