No, cross-talk is not a problem with the AcroPrep 24-well filter plates when samples are processed as recommended.

With filter plates, the term cross-talk is used when filtrate from a given well is found in adjacent wells.  In principle cross-talk can occur prior to filtration by weeping of sample from one filter plate well to another or during filtration by deposition of the filtrate in adjacent wells of the receiver plate.

As the sample wells of the AcroPrep 24-well filter plates are completely independent, there is no possibility of a sample finding its way from one well to another prior to filtration.  This, combined with the larger outlet tip-to-tip distances of the 24-well filter plate compared to those found in traditional 96-well or 384-well filter plates, means that cross-talk should not be a concern if the maximum processing volumes are adhered to.