The importance of sterile filtration and its supporting claims. Learn how prefiltration can actually improve final, sterile filtration speed and efficiency and guidelines to selecting a filtration setup.

Ensuring the performance of sterility filtration is essential in research and pharmaceutical operations. This scientific brief reviews the two types of integrity tests — destructive and nondestructive — that are commonly used by the industry to validate sterility filter performance.

Centrifugation and filtration have been widely accepted as techniques required for clarifying complex cell cultures to recover extracellular proteins such as monoclonal antibodies (mAbs). However, these steps can be time consuming and costly for labs growing their cultures in 24-well plates. In this scientific brief we explore an alternative to the use of centrifugation/filtration/flocculation to clarify and sterilize mammalian cell cultures, through an assessment of a new 24-well clarification and sterile filtration plate format.

This scientific brief reviews studies that were conducted to review the performance of black polyethersulfone (PES) membrane and several other commonly used membrane materials per the International Standard ISO 11731, “Water quality – Enumeration of Legionella.” The membrane materials were tested to determine their recovery performance for L. pneumophila and L. anisa.

A Scientific & Technical Report: Diafiltration: A Fast, Efficient Method for Desalting, or Buffer Exchange of Biological Samples.  This article will cover the concepts of protein concentration and diafiltration. It will compare different ways of performing diafiltration and their impact on process time, volume, product stability, and recovery.

This scientific brief reviews new alternatives for Low- and High-Throughput RNA Isolation/Extraction 

Science Brief: Understanding Differential Pressure Monitoring in Sterile Filtration

Reducing Repetitive Stress Injury and Improving Ergonomics in Microbiological Quality Control Procedures

This technical brief describes evaluation of a simple post-use decontamination process of Jumbosep centrifugal devices following concentration of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus in wastewater samples.

Case Study: Pall Laboratory Supor® PES Filters Provide Efficiency and Recovery Benefits for Laboratory’s Legionella Testing

Tracking the spread of COVID-19 is one of the most critical means available to scientists for containing and ultimately eliminating the disease. Unfortunately, tracking efforts are significantly complicated by the fact that many individuals do not report mild symptoms, or are asymptomatic. 

Every laboratory wants to perform sterile filtration at the highest throughput without damaging the membrane. To achieve that goal, users must understand two frequently overlooked parameters: filter venting and flux rate.

Environmental DNA, or eDNA, is defined in the traditional sense as the mixture of genetic material released from an organism into its environment. Taking advantage of the fact that organisms can shed DNA via skin, hair, scales, feces, or bodily fluids as they move through their aquatic or terrestrial environment1, eDNA analysis has revolutionized how field biologists detect endangered species. For example, while a particular species of fish may no longer be physically present in an environmental sample taken from a stream or river, the fact that it has migrated through and left traces of genetic material behind allows scientist to detect it. Diluted as such, eDNA is often present in only vanishingly small quantities. It is only in the last decade that novel molecular techniques for detection and analysis have been available. To now be able to trace endangered species and problem invasive species in the wild has led to a spectacular development of eDNA studies. As the field explodes, the definition of eDNA has also evolved, with a recent call from scientists to expand the definition of eDNA to include the genetic material, both intra‐ and extracellular DNA, of microbial and macrobial species, isolated from an environmental sample2.