Plasma Separation in Diagnostics
Date: 10. März 2021
The Vivid™ Plasma Separation membrane utilizes a patented process where a highly asymmetric membrane is specifically engineered for the generation of plasma from whole blood. This one step separation process negates the need for centrifugation.
Optimized, highly efficient membrane for one-step plasma separation from whole blood without the use of centrifugation.
- High Plasma Yield
- Achieve high quality plasma in less than two minutes with ≥ 80% plasma yield.
- Low Analyte Binding
- Low, non-specific binding of common diagnostic biomarkers and target analytes.
- Dependable Performance
- Efficient removal of the cellular components of whole blood, including red cells, white cells, and platelets.
- Low Lysis
- Hemolysis levels significantly lower than glass fiber media generated plasma.
- Device Integration
- Compatible with POC and POU diagnostic platforms such as lateral flow test strips and microfluidics.
The highly asymmetric nature of the Vivid™ PS membrane allows the cellular components of the blood (red cells, white cells, and platelets) to be captured in the larger pores without lysis, while the plasma flows down into the smaller pores on the downstream side of the membrane. This rapid separation process yields plasma similar in HPLC and SDS PAGE profiles to traditional centrifuged plasma in less than two minutes.
Non-specific binding of clinically relevant biomarkers is a concern when working with porous materials in diagnostic applications. Whole blood processed through the Vivid™ PS membrane has shown equivalent 2DE protein profiles for the cardiac biomarker Troponin I as compared to centrifuged plasma. This data indicates that the protein concentration of clinical biomarkers is not reduced when processed through the membrane, thus making it an ideal material for diagnostic applications.
The Vivid™ PS membrane provides high plasma yields, lowering the amount of starting whole blood needed. For point-of-care (POC) and point-of-use (POU) diagnostic applications, this is advantageous as whole blood volumes can be minimized resulting in smaller amounts of blood needed from patients or animals. The Vivid™ PS membrane can yield up to ≥80% yield of the theoretical plasma available while comparable glass fiber yields are only 30–50%.
Joseph Vickers - Portfolio Lead, Membrane & Media
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