Europe’s fastest growing bioprocessing meeting is returning to Barcelona on 22-24 March 2022, with more content, ground breaking science and practical case studies than ever before.
This eight-track conference covers the full spectrum of bioprocessing and modalities with dedicated sessions on upstream and downstream processing, cell and gene therapy CMC and manufacturing, and the latest developments in analytics, stability, and formulation.
Along with a unique set of unpublished case studies and learnings, the Summit also features educational Short Courses, Interactive Breakout Sessions, and a comprehensive exhibit hall showcasing the latest technologies, solutions and services supporting the rapidly growing bioprocessing industry.
Presentations & Posters
Join our Bioprocess Specialist Xenia Massana during the different posters sessions at Bioprocessing Summit Europe 2022
March 22-24, 2022
"Empty and Full Separation of Adeno-Associated Virus Vectors by Anion Exchange Membrane Chromatography"
The approval of adeno-associated virus (AAV) gene therapies has led to increased research into AAV production and the burgeoning of promising clinical trials. However, significant challenges persist in AAV purification as AAV harvests typically contain a majority population of empty capsids that generate an immune response without delivering the therapeutic payload. In addition, subtle differences between therapies restrict platformability.
Here we assess the performance of anion exchange (AEX) membrane chromatography as the polishing stage of an AAV platform process following affinity purification. Utilizing a novel 1 mS/cm step gradient approach, we are able to demonstrate separation of empty and full AAV capsids of serotypes 5, 8, and 9 with the Mustang® Q XT membrane. This process maximizes the high flow rate benefits of membrane chromatography relative to traditional column chromatography, while providing improved separation. Distinct populations in the UV 260/280 chromatogram, analytical trends with PCR and ELISA tests, and capsid standards prepared by ultracentrifugation reaffirm separation. This technique is scalable between the 0.86 mL Mustang Q XT Acrodisc® unit and 5 mL Mustang Q XT capsule, and effectively clears residual host cell protein and DNA contaminants.
Bioprocess Specialist, Pall Corporation, Spain
Develop your expertise at your own convenience, with 24/7 access to online learning materials on upstream, downstream processing, and Formulation & Filling. Accelerator℠ e-Training Center will support you throughout the journey, with visual resources that turn complex operations into step-by-step instructional videos, interactive training courses, and knowledge checks. Learn more about Pall's training solutions www.accelerator-training.com