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Filter Plate Solutions: Top 10 Questions Answered

Top 10 Filter Plate Questions

August 5, 2021

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Pall supplies 24, 96, and 384-well filter plates in various media and membranes that can be used across a wide range of life science and analytical applications. Identifying the correct filter plate to match your specific applications, sample type, processing needs and plate format can be a daunting task. In a recent webinar, Lori Euler, Product Manager for our Molecular Portfolio, identified how to choose the best filter plate for your specific application, creating higher efficiencies and greater productivity in your workflow.

 

In this blog post, Lori Euler answers the most common questions asked about multi-well filter plates. Lori Euler has over 20 years of experience in working in research and development in the areas of microbiology and molecular biology. Lori has extensive publications, presentations, and patents in her name, detailing her protocol and process development in the areas of metabolic engineering and bacterial strain analysis.

 

What is the best way to process filter plates? Centrifuge? Vacuum?

 

One big advantage of the AcroPrep™ filter plate family is that they can be processed in a variety of ways, allowing the plates to easily fit into any workflow. A centrifuge is recommended for applications that require collection of the filtrate, otherwise, the vacuum manifold is the best way to process filter plates. The AcroPrep filter plates are also compatible with positive pressure devices, which many labs are switching to for high throughput applications. 

 

Are processing parameters the same for all plates? (ie. will it take the same time, require the same speed, etc)

 

The typical time to process samples will vary with membrane type used, membrane pore size, application and sample characteristics. Below is a guide on typical times to process for various filter plates:

 

   

Typical Processing Time

Product

Plate Type

VacuumManifold

CentrifugeDevice

AcroPrep™ 24-well Filter Plates

0.1 µm Supor® membrane

2 minutes

12 minutes

0.45 µm Supor membrane

< 1 minute

< 10 minutes

0.8 µm Supor membrane

< 1 minute

< 10 minutes

1.2 µm Supor membrane

< 1 minute

< 10 minutes

5 µm Supor membrane

< 1 minute

< 10 minutes

1K Omega™ membrane

165 minutes

170 minutes

3K Omega membrane

135 minutes

135 minutes

10K Omega membrane

85 minutes

70 minutes

30K Omega membrane

1 hour

1 hour

50K Omega membrane

1 hour

1 hour

100K Omega membrane

30 minutes

100 minutes

AcroPrep Advance 96-Well Filter Plates for Multiplexing

PN 8049, 8019, 8029, 8027

2 seconds

< 2 minutes

AcroPrep Advance 96-Well Filter Plates for Protein Purification

PN 8029, 8039, 8129, 8130

2 seconds

< 2 minutes

PN 8071, 8072, 8171, 8172

14 seconds

< 2 minutes

AcroPrep Advance 96-WellFilter Plates for Lysate Clearance

PN 8040

< 2 seconds

< 2 minutes

PN 8075

< 9 seconds

< 2 minutes

PN 8175

< 35 seconds

< 2 seconds

PN 8275

< 60 seconds

< 2 seconds

AcroPrep Advance 96-Well Filter Plates for DNA Purification

PN 8032, 8132

2 seconds

< 2 minutes

AcroPrep Advance 96-Well Filter Plates for Ultrafiltration

3K Omega membrane

 

45 minutes

10K Omega membrane

20 minutes

8 minutes

30K Omega membrane

8 minutes

8 minutes

100K Omega membrane

4 minutes

5 minutes

AcroPrep Advance 96-Well Filter Plates for Solvent Filtration

0.2 µm PTFE

52 seconds

< 2 minutes

0.45 µm PTFE

19 seconds

< 2 minutes

AcroPrep Advance 96-Well Filter Plates for Aqueous Filtration

0.2 μm Supor

9 seconds

 

0.45 μm Supor

5 seconds

 

1.2 μm Supor

2 seconds

 

1.0 μm Glass Fiber

2 seconds

 

30-40 μm PP/PE

1 second

 

 

I have never seen a 24-well filter plate.  How would I fit it into my workflow?

 

The key to the 24-well filter plates is the volume you can process with them. If you are growing in a 24-well culture plate, you can transfer your sample directly into the 24-well filter plates for sample processing. If you are growing in other vessels, such as shake flasks, and need to process multiple samples in the 2-7 mL range these plates are an ideal solution versus using individual spin devices. You can save time, improve recovery, and reduce contamination risk.

 

Do I have to use the whole plate at once?

 

No! While that is ideal because otherwise extra care needs to be taken with the unused wells, you can use just the wells you need. It is best practice to use a sealing film to cover the wells you are not using. 

 

Is the 24-well filter plate different from a deep well plate?

 

Deep well plates typically have a solid bottom. Whereas, the AcroPrep filter plates have filters and are used with a solid bottom plate. Our 24-well filter plates come with a solid bottom receiver plate but our 96 and 384-well plates do not. Because all of our plates conform to the ANSI/SLAS microplate standard, you can use any standard solid bottom plate with these.  

 

Is there a guide to choosing the correct filter plate based on my application?

 

Product

Membrane

Application

AcroPrep™ 24-well Filter Plates

Supor

Protein Purification
Lysate Clearance
General Sample Prep
Multiplexing Assays
Aqueous Filtration

Omega

Concentration and purification of peptides, proteins, oligonucleotides, DNA, and RNA
Clean-up of labelling and PCR reactions
Desalting and buffer exchange
Fractionation based on size exclusion

PP/PE

Gross particulate clean up; i.e. plant and cannabis applications

Supor EKV

Clarification of raw cell cultures and sterile filtration of proteins and other biomolecules

AcroPrep Advance 96-Well Filter Plates for Multiplexing

PP/PE non-woven and Supor

Bead-based multiplexing assays
Flow cytometry

AcroPrep Advance 96-Well Filter Plates for Protein Purification

Supor

High throughput protein purification
Screening of chromatography sorbents
Screening of chromatography conditions
Protein fractionation
Antibody purification

AcroPrep Advance Filter Plates for Lysate Clearance

3 μm glass fiber/0.2 μm Supor membrane and 3 μm glass fiber/1.2 μm Supor membrane

Removal of bacterial debris prior to plasmid purification
Removal of bacterial and cellular debris prior to protein purification
Clearance of gross particulates

AcroPrep Advance 96-Well Filter Plates for DNA Purification

DNA binding (borosilicate glass without binder) media

Plasmid DNA purification
Genomic DNA purification

AcroPrep Advance 96-Well Filter Plates for Ultrafiltration

Omega

Size exclusion
PCR clean-up
Nucleic acid purification
Protein separation

AcroPrep Advance 96-Well Filter Plates for Solvent Filtration

PTFE

Metabolic studies
Molecular or drug synthesis reactions
Sample prep using solvents
Aggressive filtration applications

AcroPrep Advance 96-Well Filter Plates for Aqueous Filtration

PP/PE non-woven, glass fiber and Supor

General sample prep
Gross fractionation
Cell harvesting
Cell-based assays

 

Is there a guide based on application for the 24-well filter plate?

 

In general, the same applications apply for 24-well plates as for 96 and 384-well plates. The most unique application, only available in a 24-well format, is our cell clarification and sterile filtration plate. This plate combines a depth media with a sterilizing grade membrane to allow you to combine two steps in one. This plate allows you to be one and done!

 

AcroPrep™ 24-well Filter Plate with Omega™ Membrane

MWCO

Application

1K-100K

Biomolecule research, available in in a wide range of molecular weight cut off (MWCO). High recovery of specific biomolecules

AcroPrep24-well PP/PE Filter Plate

Pore Size

Application

30-40 μm

Gross particulate clean-up; i.e. plant and cannabis applications

AcroPrep24-well Filter Plate with Supor™ Membrane

Pore Size

Application

0.8-5.0 μm

Sample clean-up, particulate removal, pre-filtration; various cell-based assays, resin screening, bead-based assays.

0.45 μm

Large virus filtration, i.e use in cell and gene therapy workflows.

0.65/0.2 μm*

Sterile filtration, ideal for cell culture & protein purification needs, ensure sterile effluent. Dual layer Supor EKV membrane

0.1 μm

Mycoplasma reduction and sterile filtration for cell culture media and feeds

AcroPrep24 Clarify and Sterile Filter Plate

Pore Size

Application

Depth media over 0.65/0.2 μm

Clarification of raw cell cultures and sterile filtration of proteins and other biomolecules in one filter plate.

 

Which plate has better binding capacity in 96-well and 384-well plate for DNA and RNA?

 

Pall has several filter plates that contain glass fibre membranes. Which plate to use depends on your application and starting material. We have several application notes that can walk you through performance based on sample type and nucleic acid desired.

 

Do you have to buy special equipment for the 24-well filter plates?

 

No need! These plates were designed to meet ANSI /SLAS microplate standards so you can use any of the equipment you already have in your late that works with standard sized multi-well plates. While the footprint of the plates is standard, the heights do vary so always best to confirm the height compatibility with your centrifuge or liquid handling system.  

 

I’d like to try before I buy. Can Pall send me a free sample?

 

Yes! You can request a free sample.

 

Watch the Filter Plate Solutions webinar on demand. Learn more about AcroPrep filter Plates

 

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