Next-generation sequencing is a technology used to determine the order of nucleotides in entire genomes or targeted regions of DNA and RNA. The most reliable method for producing pure DNA for NGS library production is organic extraction, which is time-consuming and employs hazardous chemicals. Since the centrifugation steps are in individual microfuge tubes, they must be processed in batches of 10-24 samples at a time with many pipetting steps. Often, a number of sample extractions fail to produce sufficient quantity after the ethanol precipitation and must be repeated. As an alternative to organic extraction, Dr. Rhoads of the University of Arkansas explains the use of Pall® AcroPrep™ 96-well filter plates, as a method that proved cost-effective, provided consistent results, and allowed the processing of 96 samples in less than 2 hours.
Douglas Rhoads, Ph.D.
Professor – University of Arkansas
Director of Interdisciplinary Graduate Program in Cell and Molecular Biology
Jorgen de Haan, Ph.D.
Senior Applications Scientist