Protein Purification

Cost-effective and innovative chromatographic purification platforms

Many tools and techniques are available today for protein purification. Development of a purification process requires an analysis of various strategies to isolate a protein of interest. Protein purification from a complex sample, such as cell culture or serum, requires more than one chromatographic step. Typically the first step is ion exchange (IEX) or mixed-mode chromatography to fractionate the sample and yield a subpopulation of proteins containing the target molecule. This is followed by subsequent chromatographic steps to further fractionate the sub-population until a desired purity level is reached. These steps can include affinity purification and size exclusion chromatography, as well as IEX or mixed-mode chromatography to isolate the target protein.

 

Each step in the purification process requires optimization in order to maximize yield and purity of the target protein. Thus, small-scale purification experiments are used during the development and optimization phase to preserve precious sample while providing crucial information. The methods demonstrate the use of AcroPrep™ Advance 96-well filter plates combined with Pall chromatography media as an efficient tool for fractionation of small-volume protein samples. The format can be used to develop protein purification strategies and/or as a platform for moderate to high throughput needs.

 

AcroPrep Advance 96-well filter plates offer improved consistency and reproducibility;

 

  • Well design increases well-to-well consistency for sample processing and recovery.
  • Outlet tips minimize the formation of hanging drops, reducing the chance of cross contamination following filtration.
  • Optimized membrane configurations for assays, such as multiplexing.
  • Individually sealed membranes and biologically inert housing minimizes the possibility of sample cross-contamination or loss.
  • Designed to meet SBS/ANSI guidelines, ensuring compatibility with standard processing equipment.
  • Smooth top surface, textured window for labelling, and A1 corner notch allow for easy identification.
AcroPrep™ 96-well Filter Plates, 1 mL

Literature Library

  • Technical Report: Optimizing Chromatography Conditions Using AcroPrep™ Advance 96-Well Filter Plates

    Enter your details to download

    Country
    • Afghanistan
    • Albania
    • Algeria
    • American Samoa
    • Andorra
    • Angola
    • Anguilla
    • Antarctica
    • Antigua and Barbuda
    • Argentina
    • Aruba
    • Australia
    • Austria
    • Bahamas
    • Bahrain
    • Bangladesh
    • Barbados
    • Belgium
    • Belize
    • Benin
    • Bermuda
    • Bhutan
    • Bolivia
    • Botswana
    • Brazil
    • British Indian Ocean Territory
    • Brunei Darussalam
    • Bulgaria
    • Burkina Faso
    • Burundi
    • Cambodia
    • Cameroon
    • Canada
    • Cape Verde
    • Cayman Islands
    • Chad
    • Chile
    • China
    • Christmas Island
    • Cocos (Keeling) Islands
    • Colombia
    • Comoros
    • Cook Islands
    • Costa Rica
    • Croatia
    • Cuba
    • Cyprus
    • Czech Republic
    • Denmark
    • Djibouti
    • Dominica
    • Dominican Republic
    • Ecuador
    • Egypt
    • El Salvador
    • Equatorial Guinea
    • Eritrea
    • Estonia
    • Ethiopia
    • Falkland Islands
    • Faroe Islands
    • Fiji
    • Finland
    • France
    • French Guiana
    • French Polynesia
    • French Southern Territories
    • Gabon
    • Gambia
    • Germany
    • Ghana
    • Gibraltar
    • Greece
    • Greenland
    • Grenada
    • Guadeloupe
    • Guam
    • Guatemala
    • Guinea
    • Guinea-Bissau
    • Guyana
    • Haiti
    • Honduras
    • Hong Kong
    • Hungary
    • Iceland
    • India
    • Indonesia
    • Iraq
    • Ireland
    • Israel
    • Italy
    • Jamaica
    • Japan
    • Jordan
    • Kenya
    • Kiribati
    • Kuwait
    • Laos
    • Latvia
    • Lebanon
    • Lesotho
    • Liberia
    • Libya
    • Liechtenstein
    • Lithuania
    • Luxembourg
    • Macao
    • Madagascar
    • Malawi
    • Malaysia
    • Maldives
    • Mali
    • Malta
    • Marshall Islands
    • Martinique
    • Mauritania
    • Mauritius
    • Mayotte
    • Mexico
    • Monaco
    • Mongolia
    • Montserrat
    • Morocco
    • Mozambique
    • Myanmar
    • Namibia
    • Nauru
    • Nepal
    • Netherlands
    • Netherlands Antilles
    • New Caledonia
    • New Zealand
    • Nicaragua
    • Niger
    • Nigeria
    • Niue
    • Norfolk Island
    • Northern Mariana Islands
    • Norway
    • Oman
    • Pakistan
    • Palau
    • Palestinian Territory
    • Panama
    • Papua New Guinea
    • Paraguay
    • Peru
    • Philippines
    • Poland
    • Portugal
    • Puerto Rico
    • Qatar
    • Romania
    • Rwanda
    • Samoa
    • Saudi Arabia
    • Senegal
    • Seychelles
    • Sierra Leone
    • Singapore
    • Slovakia
    • Slovenia
    • Solomon Islands
    • Somalia
    • South Africa
    • Spain
    • Sri Lanka
    • St Kitts and Nevis
    • St Lucia
    • Sudan
    • Suriname
    • Swaziland
    • Sweden
    • Switzerland
    • Taiwan
    • Thailand
    • Togo
    • Tonga
    • Trinidad and Tobago
    • Tunisia
    • Turkey
    • Tuvalu
    • Uganda
    • United Arab Emirates
    • United Kingdom
    • United States
    • Uruguay
    • Vanuatu
    • Venezuela
    • Vietnam
    • Virgin Islands
    • Western Sahara
    • Yemen
    • Zambia
    • Zimbabwe

    By clicking the Submit button below and proceeding I confirm that I have reviewed and agree with the Terms of Use and the Privacy Policy.