The importance of sterile filtration and its supporting claims. Learn how prefiltration can actually improve final, sterile filtration speed and efficiency and guidelines to selecting a filtration setup.

Ensuring the performance of sterility filtration is essential in research and pharmaceutical operations. This scientific brief reviews the two types of integrity tests — destructive and nondestructive — that are commonly used by the industry to validate sterility filter performance.

Centrifugation and filtration have been widely accepted as techniques required for clarifying complex cell cultures to recover extracellular proteins such as monoclonal antibodies (mAbs). However, these steps can be time consuming and costly for labs growing their cultures in 24-well plates. In this scientific brief we explore an alternative to the use of centrifugation/filtration/flocculation to clarify and sterilize mammalian cell cultures, through an assessment of a new 24-well clarification and sterile filtration plate format.