Root Causes and Simple Solutions For False Positives in Lateral Flow Assays

Guidelines to identify and resolve false positives in lateral flow assays

August 4, 2022

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There is no singular root cause for false positives. In fact, there can be multiple factors at play depending on your specific application.

 

The problem should be approached systematically changing one parameter at a time allowing a process of elimination. Following is a proposed sequence of questions:

 

1. Is the problem due to charge? Varying the pH (from 5 - 11) will show if a positive charge is occurring and attracting the gold to the capture line.

 

2. Is the problem due to hydrophobicity? This can occur in the solid phase, capture line or gold conjugate. Varying surfactant concentrations will give an idea as to whether the problem is due to hydrophobicity problems.

 

3. Is the problem attraction between the gold and SH (thiol group). This could be from cysteine present in the capture line.

 

 

This short informative video outlines insights and provides simple solutions for resolving false positive issues.

 

 

If you have questions related to this content or other filtration concepts or how they apply to your specific application, please contact us.

 

 

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Jacques Hestres, Product Manager for Media – Membrane at Pall Corporation

Focusing primarily in Medical OEM applications. Throughout his over 25 years at Pall, Jacques has held various positions in Customer and Technical Service, Operations Planning, and Product Management. This experience has given him the knowledge of the products, the manufacturing processes used to produce these materials, and an understanding of applications where these are used. Jacques holds a Bachelor’s of Science Degree in Biology from the University of Puerto Rico.
Focusing primarily in Medical OEM applications. Throughout his over 25 years at Pall, Jacques has held various positions in Customer and Technical Service, Operations Planning, and Product Management. This experience has given him the knowledge of the products, the manufacturing processes used to produce these materials, and an understanding of applications where these are used. Jacques holds a Bachelor’s of Science Degree in Biology from the University of Puerto Rico.
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Antoine Pozniak - Product Manager Media - Membrane at Pall Corporation

Antoine has held various roles in sales and marketing throughout his 4-year career in the medical device industry. Throughout those 4 years he has worked with Key Opinion Leaders and developed commercial and technical expertise. Antoine holds an MBA from Rice University and Bachelor of Arts/Sciences in Interdisciplinary Social Sciences from The University of South Florida.
Antoine has held various roles in sales and marketing throughout his 4-year career in the medical device industry. Throughout those 4 years he has worked with Key Opinion Leaders and developed commercial and technical expertise. Antoine holds an MBA from Rice University and Bachelor of Arts/Sciences in Interdisciplinary Social Sciences from The University of South Florida.
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