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Filter Plate Solutions: Top 10 Questions Answered

Top 10 Filter Plate Questions

August 5, 2021

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Pall supplies 24, 96, and 384-well filter plates in various media and membranes that can be used across a wide range of life science and analytical applications. Identifying the correct filter plate to match your specific applications, sample type, processing needs and plate format can be a daunting task. In a recent webinar, Lori Euler, Product Manager for our Molecular Portfolio, identified how to choose the best filter plate for your specific application, creating higher efficiencies and greater productivity in your workflow.

In this blog post, Lori Euler answers the most common questions asked about multi-well filter plates. Lori Euler has over 20 years of experience in working in research and development in the areas of microbiology and molecular biology. Lori has extensive publications, presentations, and patents in her name, detailing her protocol and process development in the areas of metabolic engineering and bacterial strain analysis.

What is the best way to process filter plates? Centrifuge? Vacuum?

One big advantage of the AcroPrep™ filter plate family is that they can be processed in a variety of ways, allowing the plates to easily fit into any workflow. A centrifuge is recommended for applications that require collection of the filtrate, otherwise, the vacuum manifold is the best way to process filter plates. The AcroPrep filter plates are also compatible with positive pressure devices, which many labs are switching to for high throughput applications.

Are processing parameters the same for all plates? (ie. will it take the same time, require the same speed, etc)

The typical time to process samples will vary with membrane type used, membrane pore size, application and sample characteristics. Below is a guide on typical times to process for various filter plates:

		Typical Processing Time
Product	Plate Type	VacuumManifold	CentrifugeDevice
AcroPrep™ 24-well Filter Plates	0.1 µm Supor® membrane	2 minutes	12 minutes
	0.45 µm Supor membrane	< 1 minute	< 10 minutes
	0.8 µm Supor membrane	< 1 minute	< 10 minutes
	1.2 µm Supor membrane	< 1 minute	< 10 minutes
	5 µm Supor membrane	< 1 minute	< 10 minutes
	1K Omega™ membrane	165 minutes	170 minutes
	3K Omega membrane	135 minutes	135 minutes
	10K Omega membrane	85 minutes	70 minutes
	30K Omega membrane	1 hour	1 hour
	50K Omega membrane	1 hour	1 hour
	100K Omega membrane	30 minutes	100 minutes
AcroPrep Advance 96-Well Filter Plates for Multiplexing	PN 8049, 8019, 8029, 8027	2 seconds	< 2 minutes
AcroPrep Advance 96-Well Filter Plates for Protein Purification	PN 8029, 8039, 8129, 8130	2 seconds	< 2 minutes
	PN 8071, 8072, 8171, 8172	14 seconds	< 2 minutes
AcroPrep Advance 96-WellFilter Plates for Lysate Clearance	PN 8040	< 2 seconds	< 2 minutes
	PN 8075	< 9 seconds	< 2 minutes
	PN 8175	< 35 seconds	< 2 seconds
	PN 8275	< 60 seconds	< 2 seconds
AcroPrep Advance 96-Well Filter Plates for DNA Purification	PN 8032, 8132	2 seconds	< 2 minutes
AcroPrep Advance 96-Well Filter Plates for Ultrafiltration	3K Omega membrane		45 minutes
	10K Omega membrane	20 minutes	8 minutes
	30K Omega membrane	8 minutes	8 minutes
	100K Omega membrane	4 minutes	5 minutes
AcroPrep Advance 96-Well Filter Plates for Solvent Filtration	0.2 µm PTFE	52 seconds	< 2 minutes
	0.45 µm PTFE	19 seconds	< 2 minutes
AcroPrep Advance 96-Well Filter Plates for Aqueous Filtration	0.2 μm Supor	9 seconds
	0.45 μm Supor	5 seconds
	1.2 μm Supor	2 seconds
	1.0 μm Glass Fiber	2 seconds
	30-40 μm PP/PE	1 second

I have never seen a 24-well filter plate. How would I fit it into my workflow?

The key to the 24-well filter plates is the volume you can process with them. If you are growing in a 24-well culture plate, you can transfer your sample directly into the 24-well filter plates for sample processing. If you are growing in other vessels, such as shake flasks, and need to process multiple samples in the 2-7 mL range these plates are an ideal solution versus using individual spin devices. You can save time, improve recovery, and reduce contamination risk.

Do I have to use the whole plate at once?

No! While that is ideal because otherwise extra care needs to be taken with the unused wells, you can use just the wells you need. It is best practice to use a sealing film to cover the wells you are not using.

Is the 24-well filter plate different from a deep well plate?

Deep well plates typically have a solid bottom. Whereas, the AcroPrep filter plates have filters and are used with a solid bottom plate. Our 24-well filter plates come with a solid bottom receiver plate but our 96 and 384-well plates do not. Because all of our plates conform to the ANSI/SLAS microplate standard, you can use any standard solid bottom plate with these.

Is there a guide to choosing the correct filter plate based on my application?

Product	Membrane	Application
AcroPrep™ 24-well Filter Plates	Supor	Protein Purification Lysate Clearance General Sample Prep Multiplexing Assays Aqueous Filtration
	Omega	Concentration and purification of peptides, proteins, oligonucleotides, DNA, and RNA Clean-up of labelling and PCR reactions Desalting and buffer exchange Fractionation based on size exclusion
	PP/PE	Gross particulate clean up; i.e. plant and cannabis applications
	Supor EKV	Clarification of raw cell cultures and sterile filtration of proteins and other biomolecules
AcroPrep Advance 96-Well Filter Plates for Multiplexing	PP/PE non-woven and Supor	Bead-based multiplexing assays Flow cytometry
AcroPrep Advance 96-Well Filter Plates for Protein Purification	Supor	High throughput protein purification Screening of chromatography sorbents Screening of chromatography conditions Protein fractionation Antibody purification
AcroPrep Advance Filter Plates for Lysate Clearance	3 μm glass fiber/0.2 μm Supor membrane and 3 μm glass fiber/1.2 μm Supor membrane	Removal of bacterial debris prior to plasmid purification Removal of bacterial and cellular debris prior to protein purification Clearance of gross particulates
AcroPrep Advance 96-Well Filter Plates for DNA Purification	DNA binding (borosilicate glass without binder) media	Plasmid DNA purification Genomic DNA purification
AcroPrep Advance 96-Well Filter Plates for Ultrafiltration	Omega	Size exclusion PCR clean-up Nucleic acid purification Protein separation
AcroPrep Advance 96-Well Filter Plates for Solvent Filtration	PTFE	Metabolic studies Molecular or drug synthesis reactions Sample prep using solvents Aggressive filtration applications
AcroPrep Advance 96-Well Filter Plates for Aqueous Filtration	PP/PE non-woven, glass fiber and Supor	General sample prep Gross fractionation Cell harvesting Cell-based assays

Is there a guide based on application for the 24-well filter plate?

In general, the same applications apply for 24-well plates as for 96 and 384-well plates. The most unique application, only available in a 24-well format, is our cell clarification and sterile filtration plate. This plate combines a depth media with a sterilizing grade membrane to allow you to combine two steps in one. This plate allows you to be one and done!

AcroPrep™ 24-well Filter Plate with Omega™ Membrane
MWCO	Application
1K-100K	Biomolecule research, available in in a wide range of molecular weight cut off (MWCO). High recovery of specific biomolecules
AcroPrep24-well PP/PE Filter Plate
Pore Size	Application
30-40 μm	Gross particulate clean-up; i.e. plant and cannabis applications
AcroPrep24-well Filter Plate with Supor™ Membrane
Pore Size	Application
0.8-5.0 μm	Sample clean-up, particulate removal, pre-filtration; various cell-based assays, resin screening, bead-based assays.
0.45 μm	Large virus filtration, i.e use in cell and gene therapy workflows.
0.65/0.2 μm*	Sterile filtration, ideal for cell culture & protein purification needs, ensure sterile effluent. Dual layer Supor EKV membrane
0.1 μm	Mycoplasma reduction and sterile filtration for cell culture media and feeds
AcroPrep24 Clarify and Sterile Filter Plate
Pore Size	Application
Depth media over 0.65/0.2 μm	Clarification of raw cell cultures and sterile filtration of proteins and other biomolecules in one filter plate.

Which plate has better binding capacity in 96-well and 384-well plate for DNA and RNA?

Pall has several filter plates that contain glass fibre membranes. Which plate to use depends on your application and starting material. We have several application notes that can walk you through performance based on sample type and nucleic acid desired.

Do you have to buy special equipment for the 24-well filter plates?

No need! These plates were designed to meet ANSI /SLAS microplate standards so you can use any of the equipment you already have in your late that works with standard sized multi-well plates. While the footprint of the plates is standard, the heights do vary so always best to confirm the height compatibility with your centrifuge or liquid handling system.

I’d like to try before I buy. Can Pall send me a free sample?

Yes! You can request a free sample.

Watch the Filter Plate Solutions webinar on demand. Learn more about AcroPrep filter Plates.

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