Centramate™ 500 S Tangential Flow Filtration System

Scalable Production Technology
in a Compact Benchtop System

The Centramate™ 500 S benchtop tangential flow filtration system provides biotechnology and biopharmaceutical drug developers & manufacturers with an ultra-compact portable unit for process development in ultrafiltration (UF) and microfiltration (MF) applications.

Designed for all applications requiring concentration and diafiltration of suspended microparticulates (such as cell suspensions and lysates) or dissolved solutes (such as proteins or nucleic acids), the sanitary, self-venting, fully drainable design meets the demands of cGMP environments, with full traceability of process-wetted materials. Using the Centramate 500 S system for process development, time, risk and process volumes are all minimized.

Features

• Compact Flowpath - the ultra-compact flow path of the Centramate 500 S system allows users to operate at volumes as low as 170mL (when using a single Centramate membrane cassette) enabling high concentration factors and minimizing operating volumes
• Scalable Process Technology - designed through experience, the Centramate 500 S system meets the requirements for cGMP equipment with a comprehensive qualification and documentation package, calibrated process instrumentation and hygienic design
• Direct Flow Measurement - the magnetic inductive flowmeter provides direct flow measurement during operation, making system operation safe, accurate and user- friendly
• High Yield and Product Recovery - the hygienic, completely drainable flow path of the Centramate 500 S system enables high product yield, optimum product recovery and easy cleaning
• Safety in Operation - an adjustable pressure interlock provides product and operator safety in a robust and reliable TFF system
• Pressure Independent Flow - the four-piston diaphragm pump delivers constant flow even as the system pressure increases
• Data Logging Capability - data from the process instrumentation can be exported to a PC or laptop computer via the USB port on the Centramate 500 S system
• Documentation Package
  • Calibration certificates for measuring instruments
  • Drawings (P&ID, Layout)
  • User manual
  • Electrical wiring documentation incl. certificates
  • Welding documentation incl. certificates
  • Supplier literature
  • 3.1 certificates for product wetted parts
  • FDA conformity (EPDM); CE declaration
  • Generic IQ documentation

Benefits

• The graduated glass vessel provides full visibility and indication of fluid level during operation, so volumes can be closely controlled
• The positive displacement diaphragm pump provides pressure independent flow, ensuring critical parameters do not vary during concentration
• Scalable process technology makes the system ideal for scale-up and scale-down studies
• Direct flow measurement with the magnetic inductive flowmeter means accurate, safe and simple operation
• With an interlock controlling the recirculation pump, operator and product safety is maximized
• To eliminate transcription and recording errors, the Centramate 500 S system has a built in data acquisition system which can be connected to a PC via a USB cable
• The data can be displayed on the screen and exported to the hard drive of a connected computer. The data can be imported easily into Microsoft* Excel*

*Microsoft and Excel are trademarks of Microsoft

Click here to download a brochure for the Centramate 500 S TFF System (PDF, 2.7 Mb)

Operating Conditions

Pressure Range	0 to 6 barg (0 to 87 psig)
Temperature Range	0° C to +45° C (32° F to 113° F)
Flow Rate	15 - 600 L/hr

Operating Volumes

Maximum Working Volume¹	5 liter (nominal)
Minimum Working Volume²	170 mL

¹ 5 liter is the maximum batch volume. Larger volumes can be processed using a fed-batch approach
² 170 mL is the minimum working volume when using a single Centramate membrane cassette. Actual minimum working volume will be dependent on the installed membrane area and should be measured

Dimensions and Weight

Dimensions	W 480 mm x L 605 mm x H 945 mm (W 19" x L 24" x H 37")
Weight	50 kg (110 lb)

Power Requirements

CM500SE Version	220 V / 50 Hz
CM500SW Version	110V / 60 Hz
Power	370 W

Component Specifications

COMPONENT	TYPE	MATERIALS	SURFACE FINISH	CAPACITY/OTHER
Cassette Holder	Centramate	316L Stainless Steel	0.8 μm Ra, electropolished	0.1 - 0.5 m² membrane area
Tie-rods & Washers	N/A	304 Stainless Steel	N/A	N/A
Nuts	N/A	Bronze	N/A	N/A
Vessel	N/A	Borosilicate Glass	N/A	5 L
Recirculation Pump	4 piston diaphragm pump	316L Stainless Steel	0.8μm Ra, electropolished	600 L/hr
Diaphragm	N/A	Santoprene*	N/A	N/A
Flowmeter	Magnetic Inductive	316L Stainless Steel	0.8μm Ra, electropolished	600 L/hr
Pressure Indicator	N/A	N/A	N/A	N/A
Chemical Seal	N/A	316L Stainless Steel	0.8μm Ra	-1 to +6 bar
Transmitter	2-wire system	N/A	N/A	4 - 20 mA
Valves	Diaphragm	316L Stainless Steel	0.8μm Ra, electropolished	N/A
Diaphragm	N/A	EPDM	N/A	N/A
Piping	Weldron, longitudinal seamed	316L Stainless Steel	0.8μm Ra, electropolished	N/A
Gaskets	½ inch sanitary	EPDM	N/A	N/A
Vessel Outlet / Inlet Tubing	N/A	Silicon	N/A	N/A
Frame	Enclosed square profile	304 Stainless Steel	N/A	N/A
Cabinet	N/A	304 Stainless Steel	N/A	IP54

* Santoprene is a trademark of Advanced Elastomer Systems

Systems Information

Description	Part Number
Centramate 500 S TFF System - 220 V / 50 Hz	CM500SE
Centramate 500 S TFF System – 110 V / 60 Hz	CM500SW

Spare Parts

Vessel
O-ring	CM005OR
Vessel lid	CM005LD
Vessel lid clamp	CM005LC
Support & clamp (including rubber)	CM005GS
Vessel inlet /outlet tubing	CM005TU
Glass Vessel	CM005GR
Components
Pressure Sensor	CM012PS
Flow Sensor	CM012FS
Drain Valve	CM012DV
System Valve	CM012SV
Pump - membrane and spare kit	PQ808X01